Complex Structure of Surgical Sciences and Technologies, IRCCS Istituto Ortopedico Rizzoli, Via Di Barbiano 1/10, 40136 Bologna, Italy.
Novagenit Srl, Mezzolombardo, Via Trento 115, 38017 Mezzolombardo-Trento, Italy.
Biomed Mater. 2021 Feb 18;16(2):025007. doi: 10.1088/1748-605X/abdbda.
Due to the increasing number of orthopedic implantation surgery and advancements in biomaterial manufacturing, chemistry and topography, there is an increasing need of reliable and rapid methods for the preclinical investigation of osseointegration and bone ingrowth. Implant surface composition and topography increase osteogenicity, osteoinductivity, osteoconductivity and osseointegration of a prosthesis. Among the biomaterials used to manufacture an orthopedic prosthesis, titanium alloy (Ti-6Al-4V) is the most used. Type I collagen (COLL I) induces cell function, adhesion, differentiation and bone extracellular matrix component secretion and it is reported to improve osseointegration if immobilized on the alloy surface. The aim of the present study was to evaluate the feasibility of an alternative ex vivo model, developed by culturing rabbit cortical bone segments with Ti-6Al-4V alloy cylinders (Ti-POR), fabricated through the process of electron beam melting (EBM), to evaluate osseointegration. In addition, a comparison was made with Ti-POR coated with COLL I (Ti-POR-COLL) to evaluate osseointegration in terms of bone-to-implant contact (BIC) and new bone formation (nBAr/TAr) at 30, 60 and 90 d of culture. After 30 and 60 d of culture, BIC and nBAr/TAr resulted significantly higher in Ti-POR-COLL implants than in Ti-POR. No differences have been found at 90 d of culture. With the developed model it was possible to distinguish the biomaterial properties and behavior. This study defined and confirmed for the first time the validity of the alternative ex vivo method to evaluate osseointegration and that COLL I improves osseointegration and bone growth of Ti-6Al-4V fabricated through EBM.
由于骨科植入手术的数量不断增加，以及生物材料制造、化学和拓扑学的进步，人们越来越需要可靠和快速的方法来进行骨整合和骨内生长的临床前研究。植入物表面的成分和拓扑结构增加了假体的成骨性、成骨诱导性、骨传导性和骨整合性。在用于制造骨科假体的生物材料中，钛合金（Ti-6Al-4V）是应用最广泛的。I 型胶原蛋白（COLL I）可诱导细胞功能、黏附、分化和骨细胞外基质成分的分泌，据报道，如果将其固定在合金表面，可改善骨整合。本研究旨在评估通过电子束熔化（EBM）工艺制造的 Ti-6Al-4V 合金圆柱体（Ti-POR）培养兔皮质骨段的替代离体模型的可行性，以评估骨整合。此外，还将 Ti-POR 与涂覆 COLL I 的 Ti-POR（Ti-POR-COLL）进行了比较，以评估在 30、60 和 90 d 培养时的骨与植入物接触（BIC）和新骨形成（nBAr/TAr）。在培养 30 和 60 d 后，Ti-POR-COLL 植入物的 BIC 和 nBAr/TAr 明显高于 Ti-POR。在培养 90 d 时未发现差异。通过开发的模型可以区分生物材料的特性和行为。本研究首次定义并证实了替代离体方法评估骨整合的有效性，以及 COLL I 可改善通过 EBM 制造的 Ti-6Al-4V 的骨整合和骨生长。
