L.-containing gel may improve diabetic wound healing by modulating MMP-2, MMP-9, and collagen levels.

BACKGROUND/AIM: Delayed wound healing in diabetic patients is a significant complication that reduces quality of life, prompting the continuous investigation of new therapeutic agents. This study designed to explore the dose-dependent effects of different parts of L. (CM), a medicinal plant traditionally used for skin disorders, on diabetic skin wounds.

MATERIALS AND METHODS

In diabetic rats, full-thickness excisional wounds were formed. CM-containing gels (aerial parts at 3%, 6%, 9% and root at 0.5%, 1%, 1.5%) were developed and applied to the wounds. After the treatment period, the rats were sacrificed, and wound healing activity was assessed macroscopically, histopathologically, and biochemically.

RESULTS

The CM-containing gels (aerial parts or root) accelerated wound closure and increased collagen, glutathione (GSH), and ascorbic acid (AA) content. Additionally, these gels reduced oxidative stress markers, and interleukin-1β (IL-1β) and IL-13 levels, while modulating the activities of matrix metalloproteinase-2 (MMP-2) and MMP-9.

CONCLUSION

CM accelerates the healing process by increasing antioxidant capacity and modulating MMP activity, and it may have dose-dependent effectiveness in diabetic wound management.