Kaltalioğlu Kaan, Gürsoy Elif Naz, Balabanli Kanuni Barbaros, Aytaç Zeki, Coşkun Cevher Şule
Programme of Occupational Health and Safety, Vocational School of Espiye, Giresun University, Giresun, Turkiye.
Department of Biology, Faculty of Science, Gazi University, Ankara, Turkiye.
Turk J Biol. 2025 Apr 21;49(4):409-420. doi: 10.55730/1300-0152.2757. eCollection 2025.
BACKGROUND/AIM: Delayed wound healing in diabetic patients is a significant complication that reduces quality of life, prompting the continuous investigation of new therapeutic agents. This study designed to explore the dose-dependent effects of different parts of L. (CM), a medicinal plant traditionally used for skin disorders, on diabetic skin wounds.
In diabetic rats, full-thickness excisional wounds were formed. CM-containing gels (aerial parts at 3%, 6%, 9% and root at 0.5%, 1%, 1.5%) were developed and applied to the wounds. After the treatment period, the rats were sacrificed, and wound healing activity was assessed macroscopically, histopathologically, and biochemically.
The CM-containing gels (aerial parts or root) accelerated wound closure and increased collagen, glutathione (GSH), and ascorbic acid (AA) content. Additionally, these gels reduced oxidative stress markers, and interleukin-1β (IL-1β) and IL-13 levels, while modulating the activities of matrix metalloproteinase-2 (MMP-2) and MMP-9.
CM accelerates the healing process by increasing antioxidant capacity and modulating MMP activity, and it may have dose-dependent effectiveness in diabetic wound management.
背景/目的：糖尿病患者伤口愈合延迟是一种严重并发症，会降低生活质量，这促使人们不断研究新的治疗药物。本研究旨在探讨传统用于治疗皮肤疾病的药用植物L.（CM）不同部位对糖尿病皮肤伤口的剂量依赖性影响。
在糖尿病大鼠身上形成全层切除伤口。制备含CM的凝胶（地上部分分别为3%、6%、9%，根部为0.5%、1%、1.5%）并应用于伤口。治疗期结束后，处死大鼠，从宏观、组织病理学和生物化学方面评估伤口愈合活性。
含CM的凝胶（地上部分或根部）加速了伤口闭合，增加了胶原蛋白、谷胱甘肽（GSH）和抗坏血酸（AA）的含量。此外，这些凝胶降低了氧化应激标志物、白细胞介素-1β（IL-1β）和白细胞介素-13水平，同时调节了基质金属蛋白酶-2（MMP-2）和基质金属蛋白酶-9的活性。
CM通过提高抗氧化能力和调节MMP活性来加速愈合过程，在糖尿病伤口管理中可能具有剂量依赖性疗效。
