Kaltalioglu Kaan, Balabanli Barbaros, Coskun-Cevher Sule
Department of Occupational Health and Safety, Vocational School of Espiye, Giresun University, 28600, Giresun, Turkey.
Department of Biology, Faculty of Science, Gazi University, 06500, Ankara, Turkey.
Iran J Pharm Res. 2020 Fall;19(4):264-274. doi: 10.22037/ijpr.2019.112411.13742.
As stated in many ethnobotany studies, genus is traditionally used in the treatment of wound healing. In this study, we aimed to investigate to time-course effects of the methanolic extract of (MEPE) on diabetic wounds. The subject of the experiments was 36 Wistar rats, divided into three main groups: non-diabetic control (NDM), diabetic control (STZ-DM), and -treated (MEPE). Diabetes was induced by streptozotocin (STZ). Full-thickness excisional skin wounds were opened in rats. The wounds were treated with root extract in the MEPE groups. The wound area, wound contraction rate, collagen, thiobarbituric-acid reactive substances (TBARs), nitric oxide (NOx), and glutathione (GSH) levels in wound tissue were determined for the evaluation of the wound healing on days 0, 3 and 7. Phenolic compounds of MEPE were determined by RP-HPLC-UV. The antioxidant properties were spectrophotometrically determined and the antibacterial properties were tested using the microwell-dilution method. Our results demonstrated that MEPE significantly increased wound contraction rate compared to the STZ-DM group on days 3 and 7. MEPE treated rats showed a statistical increase in the levels of NOx, GSH, collagen and a statistical decrease in the levels of TBARs. Our results, for the first time, may indicate that root extract improves and accelerates diabetic wound healing and also alters oxidative events.
正如许多民族植物学研究中所述，该属传统上用于伤口愈合的治疗。在本研究中，我们旨在研究[植物名称]甲醇提取物（MEPE）对糖尿病伤口的时程效应。实验对象为36只Wistar大鼠，分为三个主要组：非糖尿病对照组（NDM）、糖尿病对照组（STZ-DM）和[植物名称]甲醇提取物治疗组（MEPE）。通过链脲佐菌素（STZ）诱导糖尿病。在大鼠身上切开全层切除性皮肤伤口。MEPE组用[植物名称]根提取物处理伤口。在第0、3和7天测定伤口组织中的伤口面积、伤口收缩率、胶原蛋白、硫代巴比妥酸反应性物质（TBARs）、一氧化氮（NOx）和谷胱甘肽（GSH）水平，以评估伤口愈合情况。通过反相高效液相色谱-紫外检测法测定MEPE中的酚类化合物。用分光光度法测定抗氧化性能，并用微孔稀释法测试抗菌性能。我们的结果表明，与STZ-DM组相比，MEPE在第3天和第7天显著提高了伤口收缩率。用MEPE处理的大鼠NOx、GSH、胶原蛋白水平有统计学意义的升高，TBARs水平有统计学意义的降低。我们的结果首次表明，[植物名称]根提取物可改善和加速糖尿病伤口愈合，并改变氧化过程。
