Mirza Salma, Ahmad Malik Shoaib
Dow University of Health Sciences, Ojha, Karachi 74200, Pakistan.
Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan; H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan.
Biochim Biophys Acta Gen Subj. 2025 Mar;1869(3):130758. doi: 10.1016/j.bbagen.2025.130758. Epub 2025 Jan 4.
Microcrystal electron diffraction (MicroED) is an emerging method for the structure determination of proteins and peptides, enzyme-inhibitor complexes. Several structures of biomolecules, including lysozyme, proteinase K, adenosine receptor A2A, insulin, xylanase, thermolysin, DNA, and Granulovirus occlusion bodies, have been successfully determined through MicroED. As MicroED uses very small crystals for structure determination, therefore, it has several advantages over conventional X-ray diffraction methods. In this review article, we discussed the most recent developments in the field of MicroED and its applications for the structural determination of different types of peptides, proteins, enzymes, DNA, and enzyme-inhibitor-complexed structures.
微晶电子衍射（MicroED）是一种用于蛋白质、肽和酶-抑制剂复合物结构测定的新兴方法。包括溶菌酶、蛋白酶K、腺苷A2A受体、胰岛素、木聚糖酶、嗜热菌蛋白酶、DNA和杆状病毒包涵体在内的几种生物分子结构已通过MicroED成功测定。由于MicroED使用非常小的晶体进行结构测定，因此它比传统的X射线衍射方法具有多个优势。在这篇综述文章中，我们讨论了MicroED领域的最新进展及其在不同类型肽、蛋白质、酶、DNA和酶-抑制剂复合结构的结构测定中的应用。
