Patterson Sara S, Cai Yongyi, Yang Qiang, Merigan William H, Williams David R
Flaum Eye Institute, University of Rochester Medical Center, Rochester, NY, 14642.
Del Monte Institute for Neuroscience, University of Rochester Medical Center, NY, 14642.
bioRxiv. 2024 Dec 17:2024.12.17.628952. doi: 10.1101/2024.12.17.628952.
Adaptive optics scanning light ophthalmoscopy (AOSLO) enables high-resolution retinal imaging, eye tracking, and stimulus delivery in the living eye. AOSLO-mediated visual stimuli are created by temporally modulating the excitation light as it scans across the retina. As a result, each location within the field of view receives a brief flash of light during each scanner cycle (every 33-40 ms). Here we used calcium imaging with AOSLO to investigate the impact of this intermittent stimulation on the retinal ON and OFF pathways. Raster-scanned backgrounds exaggerated existing ON-OFF pathway asymmetries leading to high baseline activity in ON cells and increased response rectification in OFF cells.
自适应光学扫描激光检眼镜（AOSLO）能够在活体眼中实现高分辨率视网膜成像、眼动追踪和刺激传递。AOSLO介导的视觉刺激是通过在扫描视网膜时对激发光进行时间调制来产生的。因此，在每个扫描周期（每33 - 40毫秒）内，视野中的每个位置都会接收到短暂的闪光。在这里，我们使用AOSLO结合钙成像来研究这种间歇性刺激对视网膜ON和OFF通路的影响。光栅扫描背景放大了现有的ON - OFF通路不对称性，导致ON细胞的基线活动较高，OFF细胞的反应整流增加。
