Di Profio Federica, Di Martino Barbara, Lanave Gianvito, Robetto Serena, Prandi Ilaria, Capucchio Maria Teresa, Mandola Maria Lucia, Quaranta Giuseppe, Orusa Riccardo, Marsilio Fulvio, Martella Vito, Sarchese Vittorio
Department of Veterinary Medicine, Università degli Studi di Teramo, Località Piano D'Accio, 64100 Teramo, Italy.
Department of Veterinary Medicine, Università Aldo Moro di Bari, S.p. per Casamassima Km3, 70010 Bari, Italy.
Animals (Basel). 2024 Dec 16;14(24):3624. doi: 10.3390/ani14243624.
In 2022, a novel parvovirus was identified from an outbreak of fatal enteritis in weaned European hedgehogs () at a wildlife rescue center in Southern Italy. During sequence analysis, the strain was found to be closely related (90.4% nucleotide identity) to a chaphamaparvovirus (ChPV) discovered in Amur hedgehogs () during a large metaviromic investigation in game animals in China. In this study, we investigated the presence of this novel ChPV in necropsied European hedgehogs from different areas of North-Western Italy. Duodenal and liver samples collected from 194 necropsied hedgehogs were screened by using a specific quantitative PCR. A total of 38/194 animals (19.6%) tested positive, with ChPV DNA being detected in the duodenum (9.3%, 18/194), liver (7.2%, 14/194) or in both (3.1%, 6/194) tissue samples, with comparable rates and mean viral loads. The nearly full-length genome of four hedgehog ChPV strains was reconstructed. During phylogenetic analysis based on the NS1 and partial VP aa sequences, the four strains detected in this study tightly clustered with the prototype ChPVs previously identified in Amur and European hedgehogs within a potential novel candidate species of the genus .
2022年，在意大利南部一家野生动物救助中心，从断奶欧洲刺猬（）致命性肠炎疫情中鉴定出一种新型细小病毒。在序列分析过程中，发现该毒株与在中国对野生动物进行的大型宏病毒组调查期间在刺猬（）中发现的一种刺猬细小病毒（ChPV）密切相关（核苷酸同一性为90.4%）。在本研究中，我们调查了来自意大利西北部不同地区的经尸检的欧洲刺猬中这种新型ChPV的存在情况。使用特异性定量PCR对从194只经尸检的刺猬采集的十二指肠和肝脏样本进行筛查。共有38/194只动物（19.6%）检测呈阳性，在十二指肠（9.3%，18/194）、肝脏（7.2%，14/194）或两者（3.1%，6/194）组织样本中检测到ChPV DNA，感染率和平均病毒载量相当。重建了4株刺猬ChPV毒株的近乎全长基因组。在基于NS1和部分VP氨基酸序列的系统发育分析中，本研究检测到的4株毒株与先前在刺猬和欧洲刺猬中鉴定出的ChPV原型紧密聚集在一个潜在的新候选种属内。
