Sarchese Vittorio, Palombieri Andrea, Prandi Ilaria, Robetto Serena, Bertolotti Luigi, Capucchio Maria Teresa, Orusa Riccardo, Mauthe von Degerfeld Mitzy, Quaranta Giuseppe, Vacchetta Massimo, Martella Vito, Di Martino Barbara, Di Profio Federica
Department of Veterinary Medicine, Università degli Studi di Teramo, 64100 Teramo, TE, Italy.
Centro Animali Non Convenzionali (C.A.N.C.), Department of Veterinary Sciences, University of Turin, 10095 Grugliasco, TO, Italy.
Microorganisms. 2024 Jan 17;12(1):189. doi: 10.3390/microorganisms12010189.
The presence of bocaparvoviruses (BoVs) and bufaviruses (BuVs) in the European hedgehog () was investigated by screening duodenal and liver samples collected from 183 carcasses, delivered to wildlife rescue centers located in northwestern Italy. BoV DNA was detected in 15 animals (8.2%), with prevalences of 7.1% (13/183) and 2.7% (5/183) in intestine and liver samples, respectively. Upon the sequence analyses of the NS1 gene, two highly divergent BoVs (65.5-67.8% nt identities) were identified. Fourteen strains showed the highest identity (98.3-99.4% nt) to the hedgehog BoV strains recently detected in China in Amur hedgehogs (), whilst four strains were genetically related (98.9-99.4% nt identities) to the porcine BoVs identified in pigs and classified in the species , which included related viruses also found in rats, minks, shrews, and mice. BuV DNA was detected in the duodenal samples of two hedgehogs, with a prevalence rate of 1.1%. The nearly full-length genome of two BuV strains, Hedgehog/331DU-2022/ITA and Hedgehog/1278DU/2019/ITA, was reconstructed. Upon phylogenetic analysis based on the NS and VP aa sequences, the Italian hedgehog BuVs tightly clustered with the BuVs recently identified in the Chinese Amur hedgehogs, within a potential novel candidate species of the genus .
通过对从183具尸体采集的十二指肠和肝脏样本进行筛查，研究了欧洲刺猬（Erinaceus europaeus）中博卡细小病毒（BoVs）和布法病毒（BuVs）的存在情况。这些尸体被送到了位于意大利西北部的野生动物救助中心。在15只动物（8.2%）中检测到了BoV DNA，在肠道和肝脏样本中的检出率分别为7.1%（13/183）和2.7%（5/183）。对NS1基因进行序列分析后，鉴定出两种高度不同的BoV（核苷酸同一性为65.5 - 67.8%）。14个毒株与最近在中国东北刺猬（Erinaceus amurensis）中检测到的刺猬BoV毒株具有最高的同一性（核苷酸同一性为98.3 - 99.4%），而4个毒株与在猪中鉴定出并归类于该物种（包括在大鼠、水貂、鼩鼱和小鼠中也发现的相关病毒）的猪BoV在基因上相关（核苷酸同一性为98.9 - 99.4%）。在两只刺猬的十二指肠样本中检测到了BuV DNA，检出率为1.1%。重建了两个BuV毒株（刺猬/331DU - 2022/ITA和刺猬/1278DU/2019/ITA）的近乎全长基因组。基于NS和VP氨基酸序列进行系统发育分析后，意大利刺猬BuV与最近在中国东北刺猬中鉴定出的BuV紧密聚类，属于该属的一个潜在新候选物种。
