Gercek Yusuf Can, Dagsuyu Eda, Basturk Fatma Nur, Kırkıncı Seran, Yıldırım Nazlıcan, Kıskanç Gamze, Özmener Bahar, Unlu Yigit Sabri, Kalkan Seda Nur, Boztaş Kadir, Oz Gül Cevahir, Yanardağ Refiye, Bayram Nesrin Ecem, Kostić Aleksandar Ž
Department of Biology, Faculty of Science, Istanbul University, Istanbul 34116, Türkiye.
Centre for Plant and Herbal Products Research-Development, Istanbul 34116, Türkiye.
Antioxidants (Basel). 2024 Dec 5;13(12):1483. doi: 10.3390/antiox13121483.
Bee products are an important source of nutrients and bioactive phytochemicals. This study aimed to determine the chemical composition (proximate composition, general phytochemical composition, sugar, and phenolic profiles) of four different products (honey, bee pollen, bee bread, and propolis), obtained from the same apiary, as well as to assess their biological activity through antioxidant and enzyme inhibition assays (α-amylase, α-glucosidase, lipase, AchE, neuraminidase, angiotensin-converting enzyme, urease, trypsin, tyrosinase, carbonic anhydrase, thioredoxin reductase, adenosine deaminase). Clear differences were observed among the samples in terms of both chemical composition and biological activity. The analysis revealed that bee pollen exhibited the highest carbohydrate content (87.9%), while propolis was identified as the richest source of phenolic compounds (14,858.9 mg/kg) among the analyzed samples. Propolis exhibited the highest biological activity in all applied antioxidant assays (CUPRAC, DPPH, and ABTS) and in most enzyme inhibition assays. Notably, the α-glucosidase inhibition activity of propolis was comparable to that of the reference standard. In addition, honey exhibited remarkable trypsin inhibition, also comparable to the applied standard. These findings highlight the diverse bioactivities of hive products, which could play a key role in promoting health and preventing diseases.
蜂产品是营养物质和生物活性植物化学物质的重要来源。本研究旨在确定从同一养蜂场获得的四种不同产品（蜂蜜、蜂花粉、蜂粮和蜂胶）的化学成分（近似成分、一般植物化学成分、糖和酚类成分），并通过抗氧化和酶抑制试验（α-淀粉酶、α-葡萄糖苷酶、脂肪酶、乙酰胆碱酯酶、神经氨酸酶、血管紧张素转换酶、脲酶、胰蛋白酶、酪氨酸酶、碳酸酐酶、硫氧还蛋白还原酶、腺苷脱氨酶）评估它们的生物活性。在化学成分和生物活性方面，各样本之间均观察到明显差异。分析表明，蜂花粉的碳水化合物含量最高（87.9%），而在所分析的样本中，蜂胶被确定为酚类化合物最丰富的来源（14858.9毫克/千克）。在所有应用的抗氧化试验（CUPRAC、DPPH和ABTS）以及大多数酶抑制试验中，蜂胶表现出最高的生物活性。值得注意的是，蜂胶对α-葡萄糖苷酶的抑制活性与参考标准相当。此外，蜂蜜对胰蛋白酶也有显著抑制作用，同样与应用标准相当。这些发现突出了蜂产品的多种生物活性，它们在促进健康和预防疾病方面可能发挥关键作用。
