Myćka Grzegorz, Ropka-Molik Katarzyna, Cywińska Anna, Stefaniuk-Szmukier Monika
Department of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska 1 Street, 32-083 Balice, Poland.
Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Torun, Lwowska 1 Street, 87-100 Torun, Poland.
Genes (Basel). 2024 Dec 18;15(12):1618. doi: 10.3390/genes15121618.
Among numerous genes that have been a focus of equine genetic research, the (Klotho) and (Alpha-actinin-3) genes stand out due to their significant roles in muscle function and overall health, as well as performance ability. Previous studies on Arabian horses and other mammalians have shown that both and occur in different isoforms that seem to have different roles in metabolism. The main purpose of this present study was to describe different isoforms (, , , , , ) expression levels affected by the endurance effort in Arabian horses.
Blood samples were taken from a group of = 10 Arabian horses taking part in a long-distance 120 km endurance ride. After RNA isolation and reverse transcription, real-time PCR was performed. The expression levels (Relative Quantity, RQ) were calculated using the delta-delta CT method. The results showed surprisingly large differences between different isoforms expression levels which brought us to the conclusion that both and genes are suitable genetic markers to measure endurance performance. Moreover, the correlation network analyses showed that the (myo-inositol oxygenase), (SH3 domain-containing ring finger 2) and (Troponin I2, fast skeletal type) genes are significantly involved in the endurance effort metabolism.
在众多成为马遗传研究焦点的基因中，（klotho）基因和（α - 辅肌动蛋白 - 3）基因因其在肌肉功能、整体健康以及运动能力方面的重要作用而备受关注。先前对阿拉伯马和其他哺乳动物的研究表明，这两个基因均存在不同的异构体，这些异构体在新陈代谢中似乎发挥着不同的作用。本研究的主要目的是描述耐力运动对阿拉伯马不同异构体（、、、、、）表达水平的影响。
从一组n = 10匹参加120公里长途耐力赛的阿拉伯马采集血样。在进行RNA分离和逆转录后，进行实时PCR。使用ΔΔCT法计算表达水平（相对定量，RQ）。结果显示不同异构体的表达水平之间存在惊人的巨大差异，这使我们得出结论，这两个基因都是衡量耐力表现的合适遗传标记。此外，相关网络分析表明，（肌醇加氧酶）基因、（含SH3结构域的环指蛋白2）基因和（肌钙蛋白I2，快肌型）基因在耐力运动代谢中显著相关。
