675纳米处的激光发射：衰老过程的分子对抗作用。
Laser Emission at 675 nm: Molecular Counteraction of the Aging Process.
作者信息
Notari Lorenzo, Pieri Laura, Cialdai Francesca, Fusco Irene, Risaliti Chiara, Madeddu Francesca, Bacci Stefano, Zingoni Tiziano, Monici Monica
机构信息
ASA Campus Joint Laboratory, ASA Research Division, Department of Experimental and Clinical Biomedical Sciences "Mario Serio", University of Florence, 50139 Florence, Italy.
El.En. Group, 50041 Calenzano, Italy.
出版信息
Biomedicines. 2024 Nov 27;12(12):2713. doi: 10.3390/biomedicines12122713.
BACKGROUND/OBJECTIVES: Many lasers applied in skin rejuvenation protocols show emissions with wavelengths falling in the red or near-infrared (NIR) bands. To obtain further in vitro data on the potential therapeutic benefits regarding rejuvenation, we employed a 675 nm laser wavelength on cultured human dermal fibroblasts to understand the mechanisms involved in the skin rejuvenation process's signaling pathways by analyzing cytoskeletal proteins, extracellular matrix (ECM) components, and membrane integrins.
METHODS
Normal human dermal fibroblasts (NHDFs) were irradiated with a 675 nm laser 24 h after seeding, and immunofluorescence microscopy and Western blotting were applied.
RESULTS
The results demonstrate that the laser treatment induces significant changes in human dermal fibroblasts, affecting cytoskeleton organization and the production and reorganization of ECM molecules. The cell response to the treatment appears to predominantly involve paxillin-mediated signaling pathways.
CONCLUSIONS
These changes suggest that laser treatment can potentially improve the structure and function of skin tissue, with interesting implications for treating skin aging.
背景/目的：许多用于皮肤年轻化方案的激光显示出波长落在红色或近红外（NIR）波段的发射。为了获得关于年轻化潜在治疗益处的更多体外数据，我们对培养的人真皮成纤维细胞采用675nm激光波长，通过分析细胞骨架蛋白、细胞外基质（ECM）成分和膜整合素来了解皮肤年轻化过程信号通路中涉及的机制。
方法
正常人类真皮成纤维细胞（NHDFs）在接种后24小时用675nm激光照射，并应用免疫荧光显微镜和蛋白质印迹法。
结果
结果表明，激光治疗可诱导人真皮成纤维细胞发生显著变化，影响细胞骨架组织以及ECM分子的产生和重组。细胞对治疗的反应似乎主要涉及桩蛋白介导的信号通路。
结论
这些变化表明激光治疗可能改善皮肤组织的结构和功能，对治疗皮肤老化具有重要意义。
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