Magni Giada, Pieri Laura, Fusco Irene, Madeddu Francesca, Zingoni Tiziano, Rossi Francesca
Istituto di Fisica Applicata "Nello Carrara", Consiglio Nazionale delle Ricerche (CNR - IFAC), Via Madonna del Piano, 10, Sesto Fiorentino, Firenze, 50019, Italy.
El.En. Group, Via Baldanzese, 17, 50041, Calenzano, Italy.
Regen Ther. 2023 Feb 14;22:176-180. doi: 10.1016/j.reth.2023.01.007. eCollection 2023 Mar.
Light-based therapies have been proven to influence and perhaps reverse skin ageing at clinical, molecular and histologic levels. Laser technology decreases photodamage by promoting collagen type I and III synthesis and enhancing the expression of heat shock protein. : This study aims to assess different doses of 675 nm irradiation on human dermal fibroblast cells to evaluate the potential therapeutic effects on the rejuvenation process. This study employed a laser system that emits 675 nm wavelength: 260, 390, 520 and 650 J/cm doses were tested on adult human dermal fibroblast cells. Cellular viability, proliferation, and synthesis of type I and III collagen were studied. No dose tested showed effects on cell viability and proliferation at 24 and 48 h from the irradiation. Doses of 260 and 520 J/cm causes a significant decrease in type I collagen fluorescence intensity, while 390 J/cm elicits a significant increase in type III collagen expression. Our results showed that 675 nm laser irradiation does not affect cell viability while modulating cell proliferation and collagen synthesis in human adult cultured fibroblasts in vitro. These findings suggest that 675 nm laser irradiation potentially plays a role in skin rejuvenation.
基于光的疗法已被证明在临床、分子和组织学水平上能够影响甚至逆转皮肤衰老。激光技术通过促进I型和III型胶原蛋白的合成以及增强热休克蛋白的表达来减少光损伤。本研究旨在评估不同剂量的675nm辐射对人真皮成纤维细胞的影响，以评估其在皮肤年轻化过程中的潜在治疗效果。本研究采用了一种发射675nm波长的激光系统：对成人真皮成纤维细胞测试了260、390、520和650J/cm的剂量。研究了细胞活力、增殖以及I型和III型胶原蛋白的合成。在照射后24小时和48小时，所测试的剂量均未显示对细胞活力和增殖有影响。260和520J/cm的剂量导致I型胶原蛋白荧光强度显著降低，而390J/cm引起III型胶原蛋白表达显著增加。我们的结果表明，675nm激光照射在体外不影响成人培养的人成纤维细胞的细胞活力，同时调节细胞增殖和胶原蛋白合成。这些发现表明，675nm激光照射可能在皮肤年轻化中发挥作用。
