Narmuratova Meiramkul, Berillo Dmitriy, Narmuratova Zhanar, Tarlykov Pavel, Serikbayeva Assiya, Kanayat Shattyk
Department of Biotechnology, Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Al-Farabi 71, Almaty 050040, Kazakhstan.
Department of Chemical and Biochemical Engineering, Satbayev University, Satbayev 22a, Almaty 050013, Kazakhstan.
Biomedicines. 2024 Nov 27;12(12):2715. doi: 10.3390/biomedicines12122715.
Equine milk, including its whey proteins, is a source of nutrients and functional components in the human diet, and is especially beneficial for people with weakened immune systems, newborns, and athletes. Objectives Whey proteins in equine milk constitute approximately 20% of the total protein content and include various fractions such as albumin, globulin, and lactoferrin. Lactoferrin is one of the most extensively studied whey proteins in equine milk.
HPLC-Mass analysis, enzymatic hydrolysis, modeling of 3D structure and biological activity in silico.
It has antioxidant, anti-inflammatory, and immunomodulatory properties, making it a promising candidate for influencing the various aspects of cardiovascular disease pathogenesis. The products of Lactoferrin hydrolysis by trypsin were confirmed using HPLC. The half-lives of the hydrolysate in the bloodstream and in an intestine-like environment were predicted in silico. Various biological activities (antihypertensive, anti-inflammatory, and antiangiogenic) were also estimated in silico and compared with the corresponding activities of lactoferrin hydrolysate amino acid sequences from camel and dromedary milk.
The three-dimensional modeling of lactoferrin hydrolysate peptides was performed to support the development of computational models or simulations, as well as to investigate their potential antimicrobial, anti-inflammatory, or immune-modulating functions in clinical or nutritional applications.
马奶，包括其乳清蛋白，是人类饮食中营养成分和功能成分的来源，对免疫系统较弱的人、新生儿和运动员尤其有益。目的马奶中的乳清蛋白约占总蛋白含量的20%，包括白蛋白、球蛋白和乳铁蛋白等多种组分。乳铁蛋白是马奶中研究最为广泛的乳清蛋白之一。
高效液相色谱-质谱分析、酶解、三维结构建模及计算机模拟生物活性。
它具有抗氧化、抗炎和免疫调节特性，使其成为影响心血管疾病发病机制各个方面的有前景的候选物。用高效液相色谱法确认了胰蛋白酶水解乳铁蛋白的产物。在计算机上预测了水解产物在血液和类似肠道环境中的半衰期。还在计算机上评估了各种生物活性（降压、抗炎和抗血管生成），并与骆驼奶和单峰驼奶乳铁蛋白水解产物氨基酸序列的相应活性进行了比较。
对乳铁蛋白水解产物肽进行三维建模，以支持计算模型或模拟的开发，并研究其在临床或营养应用中的潜在抗菌、抗炎或免疫调节功能。
