Jabłońska-Trypuć Agata, Pankiewicz Walentyn, Wołejko Elżbieta, Sokołowska Gabriela, Estévez Jorge, Sogorb Miguel A, Wydro Urszula
Department of Chemistry, Biology and Biotechnology, Faculty of Civil Engineering and Environmental Sciences, Bialystok University of Technology, Wiejska 45E Street, 15-351 Białystok, Poland.
Academy of Medical Sciences in Białystok, Krakowska Street 9, 15-875 Białystok, Poland.
Bioengineering (Basel). 2024 Nov 25;11(12):1188. doi: 10.3390/bioengineering11121188.
Human skin fibroblasts are an excellent in vitro model for tracking the processes occurring in human skin and studying the potential impact of various biologically active substances on these processes. Two plant hormones, which are included in the cytokinins group-kinetin (K) and N-6-benzyladenine (BA)-have a positive effect on human skin. Therefore, an attempt was made to examine the effect they have on key skin functions, cell proliferation, and migration, as well as collagen synthesis in them. The effect of phytohormones was studied at selected concentrations for kinetin-10 μM and 1 μM-and for N-6-benzyladenine-1 μM and 0.1 μM. A wound-healing assay was used in order to analyze cell migration and proliferation. The content of total protein and collagen in cells and culture medium was determined. The obtained results confirm that the studied compounds induce cell migration and proliferation, as well as collagen biosynthesis. The positive effect of kinetin and N-6-benzyladenine on fibroblast metabolism that we have demonstrated allows us to indicate them as compounds with potentially therapeutic properties. Therefore, we conclude that they should be subjected to further molecular and in vivo studies focusing on pathologies connected with skin diseases and aging.
人皮肤成纤维细胞是追踪人皮肤中发生的过程以及研究各种生物活性物质对这些过程潜在影响的优秀体外模型。细胞分裂素类中的两种植物激素——激动素（K）和N - 6 - 苄基腺嘌呤（BA）——对人皮肤有积极作用。因此，人们尝试研究它们对关键皮肤功能、细胞增殖和迁移以及其中胶原蛋白合成的影响。研究了激动素（10 μM和1 μM）以及N - 6 - 苄基腺嘌呤（1 μM和0.1 μM）在选定浓度下植物激素的作用。使用伤口愈合试验来分析细胞迁移和增殖。测定了细胞和培养基中总蛋白和胶原蛋白的含量。所得结果证实，所研究的化合物可诱导细胞迁移和增殖以及胶原蛋白生物合成。我们所证明的激动素和N - 6 - 苄基腺嘌呤对成纤维细胞代谢的积极作用使我们能够将它们表明为具有潜在治疗特性的化合物。因此，我们得出结论，它们应接受进一步的分子和体内研究，重点关注与皮肤疾病和衰老相关的病理学。
