Human Skin Fibroblasts as an In Vitro Model Illustrating Changes in Collagen Levels and Skin Cell Migration Under the Influence of Selected Plant Hormones.

Human skin fibroblasts are an excellent in vitro model for tracking the processes occurring in human skin and studying the potential impact of various biologically active substances on these processes. Two plant hormones, which are included in the cytokinins group-kinetin (K) and N-6-benzyladenine (BA)-have a positive effect on human skin. Therefore, an attempt was made to examine the effect they have on key skin functions, cell proliferation, and migration, as well as collagen synthesis in them. The effect of phytohormones was studied at selected concentrations for kinetin-10 μM and 1 μM-and for N-6-benzyladenine-1 μM and 0.1 μM. A wound-healing assay was used in order to analyze cell migration and proliferation. The content of total protein and collagen in cells and culture medium was determined. The obtained results confirm that the studied compounds induce cell migration and proliferation, as well as collagen biosynthesis. The positive effect of kinetin and N-6-benzyladenine on fibroblast metabolism that we have demonstrated allows us to indicate them as compounds with potentially therapeutic properties. Therefore, we conclude that they should be subjected to further molecular and in vivo studies focusing on pathologies connected with skin diseases and aging.