Molecular Markers in Embryo Non-Development: Analysis of Gene Expressions (, , ) in Spent Embryo Culture Medium.

An embryo culture medium is a specialized set of ambient conditions, technological equipment, and nutrients that embryos require to grow properly. We aimed to investigate the , , and gene expression differences between developing and non-developing embryos in spent embryo culture medium. , , and gene expressions were determined from the spent embryo culture medium containing developing and non-developing embryos of 20 normoresponder patients admitted to the Bahçeci Umut IVF Center. An increase in gene expression ( < 0.05) and a decrease in gene expression ( < 0.001) were observed in mediums of developing compared to the non-developing embryos. No difference was observed in gene expression ( > 0.05). While there was a correlation between and genes in the non-growing group (r < 0.01); no correlation was observed in the developing group (r > 0.05). Both normoresponder groups will be similar in terms of proliferation rate. The low expression that observed high telomerase activity in embryo development maintains continuity and avoids mechanisms that result in cell death. A molecular study of the embryo development in patients with similar characteristics may help to understand the pathogenesis of the disease and establish a diagnosis and specific treatment.