Decsi Kincső, Ahmed Mostafa, Rizk Roquia, Abdul-Hamid Donia, Tóth Zoltán
Institute of Agronomy, Hungarian University of Agriculture and Life Sciences, Georgikon Campus, 8360 Keszthely, Hungary.
Festetics Doctoral School, Institute of Agronomy, Hungarian University of Agriculture and Life Sciences, Georgikon Campus, 8360 Keszthely, Hungary.
Int J Mol Sci. 2024 Dec 11;25(24):13280. doi: 10.3390/ijms252413280.
The study investigated the application of humic acids (HAs) and a combination of humic acids and amino acids (HA+AA) in maize under field conditions. Based on preliminary data in the literature, the aim was to investigate the effects of the two plant conditioning compounds on plant physiological parameters. In addition to measuring plant physiological parameters in the field, a complete transcriptome analysis was performed to determine exactly which genes were expressed after the treatments and in which physiological processes they play a role. Maize plants showed significant positive yield changes after two priming treatments. Genome-wide transcriptomic analysis revealed the activation of photosynthetic and cellular respiration processes, as well as protein synthesis pathways, which explains the increased yield even under extreme precipitation conditions. The results show that the HA treatment helped in water management and increased the chlorophyll content, while the HA+AA treatment led to higher protein and dry matter contents. The post-harvest tests also show that the HA+AA treatment resulted in the highest yield parameters. Functional annotation of the maize super transcriptome revealed genes related to translation processes, photosynthesis, and cellular respiration. The combined pathway analysis showed that the HA and combined treatments activated genes related to photosynthesis, carbon fixation, and cellular respiration, providing valuable in-depth insight into the usefulness of the HA and HA+AA treatments in priming. Based on the studies, we believe that the use of natural-based humic acid plant conditioners may provide a beneficial opportunity to promote renewable, regenerative agriculture.
该研究调查了腐殖酸（HA）以及腐殖酸与氨基酸组合（HA + AA）在田间条件下对玉米的应用效果。基于文献中的初步数据，目的是研究这两种植物调理化合物对植物生理参数的影响。除了在田间测量植物生理参数外，还进行了完整的转录组分析，以确切确定处理后哪些基因被表达以及它们在哪些生理过程中发挥作用。经过两次引发处理后，玉米植株的产量出现了显著的正向变化。全基因组转录组分析揭示了光合作用、细胞呼吸过程以及蛋白质合成途径的激活，这解释了即使在极端降水条件下产量仍能增加的原因。结果表明，HA处理有助于水分管理并增加叶绿素含量，而HA + AA处理则导致更高的蛋白质和干物质含量。收获后的测试还表明，HA + AA处理产生了最高的产量参数。玉米超级转录组的功能注释揭示了与翻译过程、光合作用和细胞呼吸相关的基因。联合通路分析表明，HA处理和联合处理激活了与光合作用、碳固定和细胞呼吸相关的基因，为深入了解HA和HA + AA处理在引发中的作用提供了有价值的见解。基于这些研究，我们认为使用天然腐殖酸植物调理剂可能为促进可再生、再生农业提供有益的机会。
