Kostadinova-Slavova Denitsa, Petkova-Parlapanska Kamelia, Koleva Irina, Angelova Mariya, Sadi J Al-Dahwi Rafaah, Georgieva Ekaterina, Karamalakova Yanka, Nikolova Galina
Obstetrics and Gynaecology Clinic, UMHAT "Prof. St. Kirkovich", 6000 Stara Zagora, Bulgaria.
Department of Medical Chemistry and Biochemistry, Medical Faculty, Trakia University, 11 Armeiska Str., 6000 Stara Zagora, Bulgaria.
Int J Mol Sci. 2024 Dec 17;25(24):13497. doi: 10.3390/ijms252413497.
The present study aimed to investigate and compare oxidative stress biomarkers and antioxidant enzyme activity in the serum of women at risk of developing preeclampsia (PE) to prevent adverse pregnancy outcomes through early intervention. Changes in soluble fms-like tyrosine kinase-1 (sFlt-1) and placental growth factor (PlGF) levels were measured between 11 and 13 gestational weeks (gw.) before the onset of preeclampsia and its associated complications. This study evaluated the feasibility of the sFlt-1/PlGF biomarker ratio in predicting preeclampsia and adverse pregnancy outcomes, with the goal of preventive therapy with acetylsalicylic acid (150 mg daily), with acetylsalicylic acid (75 mg daily) and Clampsilin. For this purpose, the following were evaluated: (1) the levels of reactive oxygen species (ROS) and reactive nitrogen species (RNS) as parameters of oxidative stress; (2) lipid oxidation; (3) antioxidant enzyme activity; and (4) cytokine production. Analysis of the results showed that pregnant women at risk of preeclampsia had significantly higher levels of ROS, lipid oxidation, and superoxide anion radical (•O) levels compared to normal pregnancies. In PE, depleted levels of nitric oxide (NO), impaired NO synthase system (NOS), and reduced antioxidant enzyme activity ( < 0.03) suggest that PE patients cannot compensate for oxidative stress (OS). In conclusion, oxidative stress in PE plays a key role, which arises from placental problems and affects both mother and baby. The groups with acetylsalicylic acid therapy (150 mg and 75 mg) were better affected compared to those on Clampsillin.
本研究旨在调查和比较有发生先兆子痫（PE）风险的女性血清中的氧化应激生物标志物和抗氧化酶活性，以通过早期干预预防不良妊娠结局。在先兆子痫及其相关并发症发作前的妊娠11至13周期间，测量可溶性fms样酪氨酸激酶-1（sFlt-1）和胎盘生长因子（PlGF）水平的变化。本研究评估了sFlt-1/PlGF生物标志物比值在预测先兆子痫和不良妊娠结局方面的可行性，目标是使用乙酰水杨酸（每日150毫克）、乙酰水杨酸（每日75毫克）和Clampsilin进行预防性治疗。为此，评估了以下内容：（1）作为氧化应激参数的活性氧（ROS）和活性氮（RNS）水平；（2）脂质氧化；（3）抗氧化酶活性；以及（4）细胞因子产生。结果分析表明，与正常妊娠相比，有先兆子痫风险的孕妇的ROS、脂质氧化和超氧阴离子自由基（•O）水平显著更高。在先兆子痫中，一氧化氮（NO）水平降低、一氧化氮合酶系统（NOS）受损以及抗氧化酶活性降低（<0.03）表明，先兆子痫患者无法代偿氧化应激（OS）。总之，先兆子痫中的氧化应激起关键作用，其源于胎盘问题，影响母婴双方。与使用Clampsilin的组相比，接受乙酰水杨酸治疗（150毫克和75毫克）的组受影响较小。
