Deng Zi, Iwasaki Kengo, Peng Yihao, Honda Yoshitomo
Department of Oral Anatomy, Osaka Dental University, Osaka 573-1121, Japan.
Advanced Medicine Research Center, Translational Research Institute for Medical Innovation (TRIMI), Osaka Dental University, Osaka 573-1121, Japan.
Int J Mol Sci. 2024 Dec 23;25(24):13745. doi: 10.3390/ijms252413745.
Recently, it has been reported that mesenchymal stem cell (MSC)-derived humoral factors promote skin wound healing. As these humoral factors are transiently stored in cytoplasm, we collected them as part of the cell extracts from MSCs (MSC-ext). This study aimed to investigate the effects of MSC-ext on skin wound healing. We examined the effects of MSC-ext on cell proliferation and migration. Additionally, the effect of MSC-ext on skin wound healing was evaluated using a mouse skin defect model. The MSC-ext enhanced the proliferation of dermal fibroblasts, epithelial cells, and endothelial cells. It also increased the number of migrating fibroblasts and epithelial cells. The skin defects treated with MSC-ext demonstrated rapid wound closure compared to those treated with phosphate-buffered saline. The MSC-ext group exhibited a thicker dermis, larger Picrosirius red-positive areas, and a higher number of Ki67-positive cells. Our results indicate that MSC-ext promotes the proliferation and/or migration of fibroblasts, epithelial cells, and endothelial cells, and enhances skin wound healing. This suggests the therapeutic potential of MSC-ext in treating skin defects as a novel cell-free treatment modality.
最近，有报道称间充质干细胞（MSC）衍生的体液因子可促进皮肤伤口愈合。由于这些体液因子短暂存储在细胞质中，我们将它们作为MSC细胞提取物（MSC-ext）的一部分进行收集。本研究旨在探讨MSC-ext对皮肤伤口愈合的影响。我们检测了MSC-ext对细胞增殖和迁移的作用。此外，使用小鼠皮肤缺损模型评估了MSC-ext对皮肤伤口愈合的影响。MSC-ext增强了真皮成纤维细胞、上皮细胞和内皮细胞的增殖。它还增加了迁移的成纤维细胞和上皮细胞的数量。与用磷酸盐缓冲盐水处理的皮肤缺损相比，用MSC-ext处理的皮肤缺损显示出更快的伤口闭合。MSC-ext组表现出更厚的真皮、更大的天狼星红阳性区域和更多的Ki67阳性细胞。我们的结果表明，MSC-ext促进成纤维细胞、上皮细胞和内皮细胞的增殖和/或迁移，并增强皮肤伤口愈合。这表明MSC-ext作为一种新型的无细胞治疗方式，在治疗皮肤缺损方面具有治疗潜力。
