Akbar Rasheed, Manzoor Sadia, Azad Rashid, Makai Gul, Rahim Junaid, Sheikh Umer Ayyaz Aslam, Ali Amjad, Aziz Tariq, Ahmad Hafiz Ishfaq, Ahmed Mukhtar, Du Daolin, Sun Jianfan
Institute of Environment and Ecology, School of Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China.
Department of Entomology, Faculty of Physical and Applied Sciences, The University of Haripur, Haripur 22062, Pakistan.
Insects. 2024 Dec 2;15(12):959. doi: 10.3390/insects15120959.
The melon fruit fly, (Coquillett) (Diptera: Tephritidae), is a notorious pest, posing a significant threat to a wide range of fruits and vegetables, leading to substantial agricultural losses worldwide. With growing concerns over chemical pesticide resistance and environmental safety, plant-based insecticides have emerged as eco-friendly and economically sustainable alternatives. In this context, the present study delves into the insecticidal potential of extracts against . The crude extract of was systematically fractionated using a series of organic solvents with increasing polarities. The fraction demonstrating the highest insecticidal activity was further purified for the isolation of bioactive compounds, employing advanced chromatographic techniques such as Column Chromatography, coupled with state-of-the-art analytical methods including Gas Chromatography-Mass Spectrometry (GC-MS) and Fourier Transform Infrared Spectroscopy (FTIR). Bioassays were conducted on using the crude extract and its fractions in n-hexane, methanol, and ethyl acetate. Among the isolated compounds, 11,14,17-Eicosatrienoic acid was identified in both the methanol and ethyl acetate fractions. This compound exhibited remarkable insecticidal efficacy, with an LC value of 1.36%, a linearity of R = 0.64, and a statistically significant probability ( < 0.01). Particularly, 11,14,17-Eicosatrienoic acid emerged as the most potent bioactive agent against highlighting its potential as a natural insecticide. These findings underscore the potential of as a valuable source of bioactive compounds for the sustainable management of . This study not only broadens the scope of plant-based pest control strategies but also opens avenues for further exploration of natural compounds in integrated pest management.
瓜实蝇（Coquillett）（双翅目：实蝇科）是一种臭名昭著的害虫，对多种水果和蔬菜构成重大威胁，在全球范围内导致大量农业损失。随着对化学农药抗性和环境安全的担忧日益增加，植物源杀虫剂已成为环保且经济可持续的替代品。在此背景下，本研究深入探讨了[植物名称]提取物对瓜实蝇的杀虫潜力。使用一系列极性递增的有机溶剂对[植物名称]的粗提物进行了系统分级分离。对表现出最高杀虫活性的级分进一步纯化以分离生物活性化合物，采用了柱色谱等先进的色谱技术，并结合了气相色谱 - 质谱联用（GC - MS）和傅里叶变换红外光谱（FTIR）等先进的分析方法。使用粗提物及其在正己烷、甲醇和乙酸乙酯中的级分对瓜实蝇进行了生物测定。在分离出的化合物中，在甲醇和乙酸乙酯级分中均鉴定出11,14,17 - 二十碳三烯酸。该化合物表现出显著的杀虫效果，LC值为1.36%，线性度R = 0.64，且具有统计学显著概率（P < 0.01）。特别是，11,14,17 - 二十碳三烯酸成为对抗瓜实蝇最有效的生物活性剂，突出了其作为天然杀虫剂的潜力。这些发现强调了[植物名称]作为瓜实蝇可持续管理中生物活性化合物宝贵来源的潜力。本研究不仅拓宽了植物源害虫控制策略的范围，还为在综合害虫管理中进一步探索天然化合物开辟了道路。
