Lan Yanyan, Li Jingpeng, Zhang Shuo, Qin Qiuju, Liu Deguang, Luo Chen, Han Shipeng, Wang Da, He Yunzhuan
College of Plant Protection, Hebei Agricultural University, Baoding 071001, China.
College of Plant Protection, Northwest A&F University, Yangling 712100, China.
Insects. 2024 Dec 11;15(12):980. doi: 10.3390/insects15120980.
, an obligate endosymbiont of most aphid species, can influence aphids' host adaptability through amino acid metabolism, potentially mediating biotype differentiation. However, its role in the biotype differentiation of remains unclear. To address this issue, six biotypes were tested in this study. abundance varied among biotypes fed on different wheat/barley varieties (i.e., Zhong 4 wumang, 186-TM12-34; Dulihuang, Zaoshu No.3, Xiyin No.2). The reduction in abundance through antibiotic (rifampicin) treatment altered the virulence of five biotypes. Based on transcriptome analysis, the differential expression of three genes (i.e., , , and ) related to leucine, tryptophan, isoleucine, and valine metabolism was detected between different biotypes. Principal component analysis showed that leucine and tryptophan deficiencies most significantly impacted nymph development duration and aphid fecundity. Additionally, a neighbor-joining phylogenetic tree indicated the genetic differentiation of among different biotypes. These results suggest -mediated amino acid metabolism is correlated with biotype differentiation in , although the precise mechanisms by which influences this differentiation require further investigation. This study can offer a theoretical basis for the development of resistant crops, leading to the sustainable control of this aphid and reduced reliance on chemical insecticides.
作为大多数蚜虫物种的专性内共生菌，可通过氨基酸代谢影响蚜虫的宿主适应性，可能介导生物型分化。然而，其在[具体蚜虫名称]生物型分化中的作用尚不清楚。为解决这一问题，本研究对六种[具体蚜虫名称]生物型进行了测试。在以不同小麦/大麦品种（即中4无芒、186 - TM12 - 34；杜丽黄、早熟3号、西引2号）为食的生物型中，[具体蚜虫名称]的丰度有所不同。通过抗生素（利福平）处理降低[具体蚜虫名称]丰度改变了五种[具体蚜虫名称]生物型的毒力。基于转录组分析，在不同生物型之间检测到与亮氨酸、色氨酸、异亮氨酸和缬氨酸代谢相关的三个基因（即[基因名称1]、[基因名称2]和[基因名称3]）的差异表达。主成分分析表明，亮氨酸和色氨酸缺乏对若虫发育持续时间和蚜虫繁殖力影响最为显著。此外，邻接法系统发育树表明不同生物型之间[具体蚜虫名称]存在遗传分化。这些结果表明，[具体蚜虫名称]介导的氨基酸代谢与[具体蚜虫名称]的生物型分化相关，尽管[具体蚜虫名称]影响这种分化的确切机制需要进一步研究。本研究可为抗性作物的开发提供理论依据，从而实现对这种蚜虫的可持续控制并减少对化学杀虫剂的依赖。
