Jain Kalpana, Prelic Sinisa, Hansson Bill S, Wicher Dieter
Department Evolutionary Neuroethology, Max Planck Institute for Chemical Ecology, 07745 Jena, Germany.
Insects. 2024 Dec 22;15(12):1016. doi: 10.3390/insects15121016.
V-ATPases are ubiquitous and evolutionarily conserved rotatory proton pumps, which are crucial for maintaining various biological functions. Previous investigations have shown that a V-ATPase is present in the support cells of moth trichoid sensilla and influences their olfactory sensory neuron performance. Generally, V-ATPases are thought to regulate the pH value within the sensillum lymph, and aid K homeostasis within the sensillum. This, in turn, could influence various mechanisms involved within the support cells, like maintaining the receptor membrane potential (receptor current), nutrient and ion transport, odorant solubility, and various signaling mechanisms. In this study, we identify V-ATPase expression and localization in the antenna using bioinformatics and immunohistochemistry. Elucidating an olfactory V-ATPase function will improve our current understanding of how support cells contribute to 's sense of smell.
V-ATP酶是普遍存在且在进化上保守的旋转质子泵，对维持各种生物学功能至关重要。先前的研究表明，V-ATP酶存在于蛾类毛形感器的支持细胞中，并影响其嗅觉感觉神经元的性能。一般认为，V-ATP酶可调节感器淋巴液的pH值，并有助于维持感器内的钾稳态。这反过来又可能影响支持细胞内涉及的各种机制，如维持受体膜电位（受体电流）、营养物质和离子运输、气味剂溶解度以及各种信号传导机制。在本研究中，我们利用生物信息学和免疫组织化学方法确定了V-ATP酶在触角中的表达和定位。阐明嗅觉V-ATP酶的功能将增进我们目前对支持细胞如何影响嗅觉的理解。
