Kızılbey Kadriye, Köprülü Elif Nur, Temür Hatice, Canım Ateş Sezen, Özer Sevil
Department of Natural Sciences, Faculty of Engineering and Natural Sciences, Acıbadem University, Istanbul 34752, Türkiye.
Division of Biomedical Engineering, Institute of Graduate Studies, Istanbul University-Cerrahpasa, Istanbul 34320, Türkiye.
Materials (Basel). 2024 Dec 11;17(24):6064. doi: 10.3390/ma17246064.
Magnetic nanoparticles have attracted significant attention in nanoscience and nanotechnology due to their unique physicochemical properties. These properties enable their great potential in various biomedical applications, such as hyperthermia, drug delivery, tissue engineering, theranostics, and lab-on-a-chip technologies. Physical and chemical methods are conventionally used for the synthesis of nanoparticles; however, due to several limitations of these methods, research focus has recently shifted towards developing clean and eco-friendly synthesis protocols while maintaining their desirable chemical and physical properties. In this study, iron oxide nanoparticles (FeNPs) were synthesized for the first time using the green synthesis method with extracts from . The structural and magnetic characterization of FeNPs was carried out using state-of-the-art techniques. The formation of FeNPs was confirmed by UV-vis spectroscopy. The morphology and size distribution were examined by a zetasizer and SEM, which showed agglomerated ring-shaped structures with a moderate size distribution among the nanoparticles. The crystalline structure and phase purity of the FeNPs were analyzed by XRD. FT-IR spectroscopy confirmed the attachment of bioactive plant molecules on the FeNP surfaces. The TGA results indicated the presence of organic molecules on the surface of the nanoparticles. Further studies including temperature-dependent magnetization and coercivity measurements were performed by PPMS and ESR, confirming the soft magnetic characteristics of synthesized FeNPs. Additionally, the dose-dependent toxicity and anti-cancerogenic effects of the FeNPs were screened towards the glioma cancer line (C6) and fibroblast cell line (L929) in vitro using an MTT assay. After 24 h of treatment, inhibitory concentration IC50 values of 26.51 µg/mL (l929) and 10.73 µg/mL (C6) were determined, respectively. These results suggest the potential of the synthesized FeNPs in developing new biocompatible systems for diagnostic and therapeutic purposes. This study contributes to the growing demand for research in nanotechnology by offering a sustainable and effective green synthesis method for FeNPs, expanding their potential applications in nanomedicine.
磁性纳米颗粒因其独特的物理化学性质在纳米科学和纳米技术领域引起了广泛关注。这些特性使其在各种生物医学应用中具有巨大潜力，如热疗、药物递送、组织工程、诊疗一体化以及芯片实验室技术等。传统上，物理和化学方法用于合成纳米颗粒；然而，由于这些方法存在若干局限性，近期研究重点已转向开发清洁且环保的合成方案，同时保持其理想的化学和物理性质。在本研究中，首次使用绿色合成方法，以[提取物名称未给出]的提取物合成了氧化铁纳米颗粒（FeNPs）。采用先进技术对FeNPs进行了结构和磁性表征。通过紫外可见光谱法确认了FeNPs的形成。使用zeta电位仪和扫描电子显微镜（SEM）检测了其形态和尺寸分布，结果显示纳米颗粒呈现团聚的环形结构，尺寸分布适中。通过X射线衍射（XRD）分析了FeNPs的晶体结构和相纯度。傅里叶变换红外光谱（FT - IR）证实了生物活性植物分子附着在FeNP表面。热重分析（TGA）结果表明纳米颗粒表面存在有机分子。通过物理性能测量系统（PPMS）和电子自旋共振（ESR）进行了包括温度依赖性磁化和矫顽力测量在内的进一步研究，证实了合成的FeNPs具有软磁特性。此外，使用MTT法在体外筛选了FeNPs对胶质瘤癌细胞系（C6）和成纤维细胞系（L929）的剂量依赖性毒性和抗癌作用。处理24小时后，分别测定了抑制浓度IC50值为26.51μg/mL（L929）和10.73μg/mL（C6）。这些结果表明合成的FeNPs在开发用于诊断和治疗目的的新型生物相容性系统方面具有潜力。本研究通过提供一种可持续且有效的FeNPs绿色合成方法，满足了纳米技术领域日益增长的研究需求，拓展了其在纳米医学中的潜在应用。
