安德鲁司（山楂）增强氯沙坦的抗高血压活性。

Andrews (Haw.) Improves Losartan's Antihypertensive Activity.

作者信息

Amatto Pedro de Padua G, Coppede Juliana da Silva, Kitanishi Carla Renata, Braga Giovana Graça, Faria Thaysa Carvalho de, Rizzi Elen, França Suzelei de Castro, Basso Fernanda, Lopes Adriana Aparecida, Carmona Fábio, Contini Silvia Helena Taleb, Pereira Ana Maria Soares

机构信息

Department of Biotechnology of Medicinal Plants, University of Ribeirão Preto, Ribeirão Preto 14096-900, Brazil.

School of Dentistry, São Paulo State University Júlio de Mesquita Filho, Araraquara 14800-060, Brazil.

出版信息

Molecules. 2024 Dec 20;29(24):6010. doi: 10.3390/molecules29246010.

DOI:10.3390/molecules29246010

PMID:39770106

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11676209/

Abstract

BACKGROUND

Cardiovascular diseases constitute one of the leading causes of morbidity and mortality worldwide. Herbal medicines represent viable alternatives to the synthetic drugs currently employed in the control of hypertension. This study aimed to isolate and identify the chemical markers of and to investigate the antihypertensive and anti-matrix metalloproteinase (MMP2) activities of an aqueous extract of the leaves.

METHODS

The main constituents of the aqueous extract of were separated by ultra-performance liquid chromatography-mass spectrometry, and their presence was identified by NMR spectroscopy. Renovascular hypertension was induced in male Wistar rats using the two-kidney one-clip method (HTN groups), while control animals (Sham groups) were submitted to Sham surgery. Six groups of 10 animals each were treated daily for eight weeks as follows: Sham 1 (carrier), Sham 2 ( extract), HTN.1 (carrier), HTN.2 ( extract), HTN 3 (losartan), and HTN 4 ( extract with losartan).

RESULTS

The main compounds of the extract were patuletin 3--(4″--acetyl-α-L-rhamnopyranosyl)-7--(3‴--acetyl-α-L-rhamnopyranoside) (), patuletin 3--α-L-rhamnopyranosyl-7--L-rhamnopyranoside (), and -caffeoyl-malic acid (), with compounds and being chemical markers of the species. Significant reductions ( < 0.05) in systolic blood pressure and MMP2 (72kDa isoform) activity were observed in the HTN 4 group.

CONCLUSIONS

The association of extract and losartan presented in vivo effects against hypertension.

摘要

背景

心血管疾病是全球发病和死亡的主要原因之一。草药是目前用于控制高血压的合成药物的可行替代品。本研究旨在分离和鉴定[植物名称]的化学标志物，并研究其叶水提取物的降压和抗基质金属蛋白酶（MMP2）活性。

方法

采用超高效液相色谱-质谱联用技术分离[植物名称]水提取物的主要成分，并用核磁共振光谱法鉴定其存在。采用双肾单夹法诱导雄性Wistar大鼠肾血管性高血压（高血压组），而对照动物（假手术组）接受假手术。将六组，每组10只动物，每天治疗8周，具体如下：假手术1组（载体）、假手术2组（[植物名称]提取物）、高血压1组（载体）、高血压2组（[植物名称]提取物）、高血压3组（氯沙坦）和高血压4组（[植物名称]提取物与氯沙坦联用）。

结果

提取物的主要化合物为紫铆亭3-O-(4″-O-乙酰基-α-L-鼠李糖吡喃糖基)-7-O-(3‴-O-乙酰基-α-L-鼠李糖吡喃糖苷)（化合物1）、紫铆亭3-O-α-L-鼠李糖吡喃糖基-7-O-L-鼠李糖吡喃糖苷（化合物2）和对香豆酰苹果酸（化合物3），化合物1和2是该植物的化学标志物。在高血压4组中观察到收缩压和MMP2（72kDa亚型）活性显著降低（P<0.05）。