Jiang Changsheng, Chen Weiying, Yang Yanan, Li Xiaojin, Jin Mengmeng, Ghonaim Ahmed H, Li Shenghe, Ren Man
Anhui Provincial Key Laboratory of Animal Nutritional Regulation and Health, College of Animal Science, Anhui Science and Technology University, Chuzhou 233100, China.
National Key Laboratory of Agricultural Microbiology, College of Animal Sciences and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
Microorganisms. 2024 Nov 22;12(12):2396. doi: 10.3390/microorganisms12122396.
Rotavirus (RV) is a significant contributor to diarrhea in both young children and animals, especially in piglets, resulting in considerable economic impacts on the global pig industry. Isoleucine (Ile), a branched-chain amino acid, is crucial for regulating nutrient metabolism and has been found to help mitigate diarrhea. This study aimed to assess the impact of isoleucine supplementation in feed on colonic barrier function, colonic microbiota, and metabolism in RV-infected weanling piglets. A total of thirty-two weaned piglets, aged 21 days, were randomly assigned to two dietary groups (each further divided into two subgroups, with eight replicates in each subgroup), receiving diets with either 0% or 1% isoleucine for a duration of 14 days. One group from each treatment was then challenged with RV, and the experimental period lasted for 19 days. The results showed that dietary Ile significantly increased the secretion of IL-4, IL-10, and sIgA in the colon of RV-infected weanling piglets ( < 0.05). In addition, Ile supplementation notably increased the expression of tight junction proteins, including Claudin-3, Occludin, and ZO-1 ( < 0.01), as well as the mucin protein MUC-1 in the colon of RV-infected weanling piglets ( < 0.05). Gut microbiota analysis revealed that dietary Ile increased the relative abundance of Prevotella and decreased the relative abundance of Rikenellaceae in the colons of RV-infected weanling piglets. Compared with the RV+CON, metabolic pathways in the RV+ILE group were significantly enriched in vitamin digestion and absorption, steroid biosynthesis, purine metabolism, pantothenate and CoA biosynthesis, cutin, suberine, and wax biosynthesis, as well as fatty acid biosynthesis, and unsaturated fatty acid biosynthesis. In conclusion, dietary Ile supplementation can improve immunity, colonic barrier function, colonic microbiota, and colonic metabolism of RV-infected weaned piglets. These findings provide valuable insights into the role of isoleucine in the prevention and control of RV.
轮状病毒（RV）是导致幼儿和动物腹泻的一个重要因素，尤其是在仔猪中，给全球养猪业带来了相当大的经济影响。异亮氨酸（Ile）是一种支链氨基酸，对调节营养代谢至关重要，并且已发现有助于减轻腹泻。本研究旨在评估在饲料中添加异亮氨酸对感染RV的断奶仔猪结肠屏障功能、结肠微生物群和代谢的影响。总共32头21日龄的断奶仔猪被随机分为两个日粮组（每组再分为两个亚组，每个亚组有8个重复），分别接受含0%或1%异亮氨酸的日粮，持续14天。然后每组中的一组用RV进行攻毒，实验期持续19天。结果表明，日粮中的异亮氨酸显著增加了感染RV的断奶仔猪结肠中IL-4、IL-10和sIgA的分泌（<0.05）。此外，添加异亮氨酸显著增加了感染RV的断奶仔猪结肠中紧密连接蛋白Claudin-3、Occludin和ZO-1的表达（<0.01），以及粘蛋白MUC-1的表达（<0.05）。肠道微生物群分析显示，日粮中的异亮氨酸增加了感染RV的断奶仔猪结肠中普雷沃氏菌的相对丰度，并降低了理研菌科的相对丰度。与RV+CON组相比，RV+ILE组的代谢途径在维生素消化与吸收、类固醇生物合成、嘌呤代谢、泛酸和辅酶A生物合成、角质、木栓质和蜡生物合成以及脂肪酸生物合成和不饱和脂肪酸生物合成方面显著富集。总之，日粮中添加异亮氨酸可以改善感染RV的断奶仔猪的免疫力、结肠屏障功能、结肠微生物群和结肠代谢。这些发现为异亮氨酸在RV预防和控制中的作用提供了有价值的见解。
