You Minru, Yang Wenwu
MOE Key Laboratory for Biodiversity Science and Ecological Engineering, School of Life Science, Fudan University, Songhu Road 2005, Shanghai 200438, China.
Microorganisms. 2024 Dec 3;12(12):2492. doi: 10.3390/microorganisms12122492.
Symbiotic microbiota significantly influence the development, physiology, and behavior of their hosts, and therefore, they are widely studied. However, very few studies have investigated the changes in symbiotic microbiota across generations. originating from the Qinghai-Tibetan Plateau were cultured through seven generations in our laboratory, and the symbiotic microbiota of were sequenced using a 16S rRNA amplicon to analyze changes in the structure and functional properties of the symbiotic microbiota of from a harsh environment to an ideal environment. We detected substantial changes in the symbiotic microbiota of across generations. For example, the genus , a member of the gamma-subclass Proteobacteria, had the highest abundance in the first generation (G1), followed by a decrease in abundance in the fourth (G4) and seventh (G7) generations. The gene functions of the microbiota in different generations of also changed significantly. The fourth generation was mainly rich in fatty acyl-CoA synthase, acetyl-CoA acyltransferase, phosphoglycerol phosphatase, etc. The seventh generation was mainly rich in osmotic enzyme protein and ATP-binding protein of the ABC transport system. This study confirms that the alterations in the structure and functional properties of the symbiotic microbiota of under changing environments are typical responses of to environmental changes.
共生微生物群对其宿主的发育、生理和行为有显著影响，因此受到广泛研究。然而，很少有研究调查共生微生物群在多代间的变化。源自青藏高原的[具体研究对象]在我们实验室中培养了七代，并使用16S rRNA扩增子对[具体研究对象]的共生微生物群进行测序，以分析[具体研究对象]的共生微生物群从恶劣环境到理想环境时其结构和功能特性的变化。我们检测到[具体研究对象]的共生微生物群在多代间发生了显著变化。例如，γ-变形菌纲的一个成员[具体菌属]在第一代（G1）中丰度最高，随后在第四代（G4）和第七代（G7）中丰度下降。[具体研究对象]不同代的微生物群的基因功能也发生了显著变化。第四代主要富含脂肪酰辅酶A合酶、乙酰辅酶A酰基转移酶、磷酸甘油磷酸酶等。第七代主要富含渗透酶蛋白和ABC转运系统的ATP结合蛋白。本研究证实，在变化的环境下，[具体研究对象]共生微生物群的结构和功能特性的改变是[具体研究对象]对环境变化的典型反应。
