Rayko Mikhail, Kravchenko Irina, Lapidus Alla
Laboratory of Cytology of Unicellular Organisms, Institute of Cytology RAS, 194064 St. Petersburg, Russia.
Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, 119071 Moscow, Russia.
Microorganisms. 2024 Dec 16;12(12):2599. doi: 10.3390/microorganisms12122599.
A whole genome sequence of a new strain of the nitrogen-fixing bacterium known for its diverse plant growth-promoting bacteria (PGPB), was obtained for the first time. The strain, designated e AT, was isolated during a soil analysis in the Chernevaya taiga of Western Siberia, a unique and fertile forest ecosystem known for its diverse plant growth-promoting bacteria (PGPB). The genome under study is fully assembled into seven circular molecules, none of which are unequivocally plasmids, with a total length of 6.94 Mb and a G + C content of 68.66%. A detailed phylogenomic analysis confirmed its placement within the genus , specifically closely related to GSF71. Functional annotation revealed genes involved in nitrogen metabolism, highlighting the potential of strain TA as a biofertilizer and plant growth-promoting agent. The findings contribute to our understanding of the genomic diversity and metabolic potential of the genus, and they are of interest for further study in the field of comparative bacterial genomics, given the strain's multi-chromosomal genome structure.
首次获得了一种固氮细菌新菌株的全基因组序列,该菌株以其多样的植物促生细菌(PGPB)而闻名。该菌株命名为e AT,是在西西伯利亚切列涅瓦亚泰加林的土壤分析过程中分离出来的,切列涅瓦亚泰加林是一个独特且肥沃的森林生态系统,以其多样的植物促生细菌(PGPB)而闻名。所研究的基因组完全组装成七个环状分子,其中没有一个明确是质粒,总长度为6.94 Mb,G + C含量为68.66%。详细的系统基因组分析证实了它在该属中的位置,特别是与GSF71密切相关。功能注释揭示了参与氮代谢的基因,突出了TA菌株作为生物肥料和植物促生剂的潜力。这些发现有助于我们了解该属的基因组多样性和代谢潜力,鉴于该菌株的多染色体基因组结构,它们对于比较细菌基因组学领域的进一步研究具有重要意义。