Popov Ilia V, Chikindas Michael L, Popov Igor V
Faculty "Bioengineering and Veterinary Medicine", Don State Technical University, Rostov-on-Don, 344000, Russian Federation.
Health Promoting Naturals Laboratory, School of Environmental and Biological Sciences, Rutgers State University, New Brunswick, NJ, 08901, USA.
Braz J Microbiol. 2025 Jul 22. doi: 10.1007/s42770-025-01740-8.
White-nose syndrome, caused by the psychrophilic fungus Pseudogymnoascus destructans, has devastated bat populations across North America. Streptomyces albidoflavus SM254 was previously reported to exhibit antifungal activity against this pathogen, but no comprehensive genomic characterization has been performed to date. Here, we analyzed 34 S. albidoflavus genomes, including the antifungal strain SM254 and 33 publicly available references, to investigate its metabolic potential and functional distinctiveness. Using pangenome reconstruction, phylogenomics, average nucleotide identity, and KEGG pathway profiling, we found that S. albidoflavus SM254 shares high nucleotide identity (> 99%) with five closely related strains but displays a unique combination of complete ethanol fermentation capacity and asparagine biosynthesis deficiency. These traits were exclusive to SM254 and may reflect adaptation to the oxygen-limited, nutrient-variable sediment environment. Functional annotation further revealed high completeness in central energy, redox, and stress-response pathways. Although direct antifungal mechanisms remain to be experimentally validated, S. albidoflavus SM254's unique metabolic profile and ecological specialization suggest potential relevance in biocontrol contexts.
白鼻综合征由嗜冷真菌毁灭拟裸球壳菌引起，已使北美各地的蝙蝠种群遭受重创。先前有报道称白色链霉菌SM254对这种病原体具有抗真菌活性，但迄今为止尚未进行全面的基因组特征分析。在此，我们分析了34个白色链霉菌基因组，包括抗真菌菌株SM254和33个公开可用的参考基因组，以研究其代谢潜力和功能独特性。通过泛基因组重建、系统发育基因组学、平均核苷酸同一性和KEGG通路分析，我们发现白色链霉菌SM254与五个密切相关的菌株具有较高的核苷酸同一性（>99%），但具有完整的乙醇发酵能力和天冬酰胺生物合成缺陷的独特组合。这些特征是SM254独有的，可能反映了其对氧气有限、营养可变的沉积物环境的适应性。功能注释进一步揭示了中心能量、氧化还原和应激反应通路的高度完整性。尽管直接的抗真菌机制仍有待实验验证，但白色链霉菌SM254独特的代谢谱和生态特性表明其在生物防治方面具有潜在的相关性。
