Phytochemical Analysis and Antimicrobial Activity of (Gaertn.) Roxb. and Retz. Fruit Extracts Against Gastrointestinal Pathogens: Enhancing Antibiotic Efficacy.

(Gaertn) Roxb. and Retz. are significant botanicals in ancient Ayurvedic medicine. They are renowned for their therapeutic properties, notably in addressing gastrointestinal (GI) diseases. These plants have undergone thorough examination related to their antibacterial, anti-inflammatory, and antioxidant properties, which make them highly efficient natural treatments for controlling gastrointestinal infections. The current research demonstrated the antibacterial efficacy of fruit extracts of and against , , , and . We performed disc diffusion and liquid microdilution experiments to evaluate the antibacterial efficacy. All extracts of and showed good antibacterial effects against and . The minimum inhibitory concentration (MIC) values ranged from 94 µg/mL to 556 µg/mL. The methanolic extracts from both plants also showed noteworthy antibacterial activity against and , with MIC values of 755 µg/mL for both. Fractional inhibitory concentration studies revealed additive interactions between some conventional antibiotics and the plant extracts when used concurrently. Liquid chromatography-mass spectrometry (LC-MS) analyses revealed that the and extracts contained various tannins including methyl gallate, propyl gallate, gallic acid, and ellagic acid. Lethality assays conducted using Kellogg nauplii indicated that all the plant extracts are non-toxic. The antibacterial properties and absence of toxicity in and fruit extracts indicate their potential for antibiotic development, warranting additional mechanistic and phytochemical studies.