美洲茄基因组辅助发现可识别马铃薯晚疫病菌效应物的免疫受体。
Solanum americanum genome-assisted discovery of immune receptors that detect potato late blight pathogen effectors.
机构信息
The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich, UK.
State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
出版信息
Nat Genet. 2023 Sep;55(9):1579-1588. doi: 10.1038/s41588-023-01486-9. Epub 2023 Aug 28.
Potato (Solanum tuberosum) and tomato (Solanum lycopersicon) crops suffer severe losses to late blight caused by the oomycete pathogen Phytophthora infestans. Solanum americanum, a relative of potato and tomato, is globally distributed and most accessions are highly blight resistant. We generated high-quality reference genomes of four S. americanum accessions, resequenced 52 accessions, and defined a pan-NLRome of S. americanum immune receptor genes. We further screened for variation in recognition of 315P. infestans RXLR effectors in 52 S. americanum accessions. Using these genomic and phenotypic data, we cloned three NLR-encoding genes, Rpi-amr4, R02860 and R04373, that recognize cognate P. infestans RXLR effectors PITG_22825 (AVRamr4), PITG_02860 and PITG_04373. These genomic resources and methodologies will support efforts to engineer potatoes with durable late blight resistance and can be applied to diseases of other crops.
马铃薯（Solanum tuberosum）和番茄（Solanum lycopersicon）作物因卵菌病原体致病疫霉（Phytophthora infestans）引起的晚疫病而遭受严重损失。马铃薯和番茄的亲缘种美洲茄（Solanum americanum）在全球范围内分布广泛，大多数品种具有高度的抗晚疫病能力。我们生成了四个美洲茄品种的高质量参考基因组，重测序了 52 个品种，并定义了美洲茄免疫受体基因的泛 NLR 组。我们进一步筛选了 52 个美洲茄品种对 315 个致病疫霉 RXLR 效应蛋白的识别变异。利用这些基因组和表型数据，我们克隆了三个 NLR 编码基因，Rpi-amr4、R02860 和 R04373，它们识别同源的致病疫霉 RXLR 效应蛋白 PITG_22825（AVRamr4）、PITG_02860 和 PITG_04373。这些基因组资源和方法将支持具有持久晚疫病抗性的马铃薯工程的努力，并可应用于其他作物的病害。
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