Alexeeva Olga V, Konstantinova Marina L, Siracusa Valentina, Podmasterev Vyacheslav V, Martirosyan Levon Yu, Karyagina Olga K, Kozlov Sergey S, Lomakin Sergey M, Tretyakov Ilya V, Petrova Tuyara V, Iordanskii Alexey L
Emanuel Institute of Biochemical Physics of Russian Academy of Sciences, 119334 Moscow, Russia.
Department of Chemical Science (DSC), University of Catania, Viale A. Doria 6, 95125 Catania, Italy.
Polymers (Basel). 2024 Dec 20;16(24):3554. doi: 10.3390/polym16243554.
Glycerol-(9,10-trioxolane) trioleate (OTOA) is a promising material that combines good plasticizing properties for PLA with profound antimicrobial activity, which makes it suitable for application in state-of-the-art biomedical and packaging materials with added functionality. On the other hand, application of OTOA in PLA-based antibacterial materials is hindered by a lack of knowledge on kinetics of the OTOA release. In this work, the release of glycero-(9,10-trioxolane) trioleate (OTOA) from PLA films with 50% OTOA content was studied during incubation in normal saline solution, and for the first time, the kinetics of OTOA release from PLA film was evaluated. Morphological, thermal, structural and mechanical properties of the PLA + 50% OTOA films were studied during incubation in normal saline and corresponding OTOA release using differential scanning calorimetry (DSC), X-ray diffraction (XRD), Fourier-transform infrared (FTIR) spectroscopy and mechanical tests. It was confirmed by DSC and XRD that incubation in the saline solution and corresponding OTOA release from PLA film does not lead to significant changes in the structure of the polymer matrix. Thus, the formation of more disturbed α' crystalline phase of PLA due to partial hydrolysis of amorphous zones and/or most unstable crystallites in the PLA/OTOA semi-crystalline structure was observed. The degree of crystallinity of PLA + OTOA film was also slightly increased at the prolonged stages of OTOA release. PLA + 50% OTOA film retained its strength properties after incubation in normal saline, with a slight increase in the elastic modulus and tensile strength, accompanied by a significant decrease in relative elongation at break. The obtained results showed that PLA + 50% OTOA film could be characterized by sustained OTOA release with the amount of released OTOA exceeding 50% of the initial content in the PLA film. The OTOA release profile was close to zero-order kinetics, which is beneficial in order to provide stable drug release pattern. Developed PLA + 50% OTOA films showed a strong and stable antibacterial effect against and , bacterial strains with multidrug resistance behavior. The resulting PLA + OTOA films could be used in a variety of biomedical and packaging applications, including wound dressings and antibacterial food packaging.
甘油 -（9,10 - 三氧杂环戊烷）三油酸酯（OTOA）是一种很有前景的材料，它兼具对聚乳酸（PLA）良好的增塑性能和显著的抗菌活性，这使其适用于具有附加功能的先进生物医学和包装材料。另一方面，由于对OTOA释放动力学缺乏了解，限制了OTOA在基于PLA的抗菌材料中的应用。在这项工作中，研究了含50% OTOA的PLA薄膜在生理盐水溶液中孵育期间甘油 -（9,10 - 三氧杂环戊烷）三油酸酯（OTOA）的释放情况，并且首次评估了OTOA从PLA薄膜中的释放动力学。使用差示扫描量热法（DSC）、X射线衍射（XRD）、傅里叶变换红外（FTIR）光谱和力学测试研究了PLA + 50% OTOA薄膜在生理盐水中孵育期间的形态、热性能、结构和力学性能以及相应的OTOA释放情况。DSC和XRD证实，在盐溶液中孵育以及PLA薄膜中相应的OTOA释放不会导致聚合物基体结构发生显著变化。因此，观察到由于PLA / OTOA半结晶结构中非晶区和/或最不稳定微晶的部分水解，导致PLA形成了更多紊乱的α'晶相。在OTOA释放的延长阶段，PLA + OTOA薄膜的结晶度也略有增加。PLA + 50% OTOA薄膜在生理盐水中孵育后保留了其强度性能，弹性模量和拉伸强度略有增加，同时断裂伸长率显著降低。所得结果表明，PLA + 50% OTOA薄膜的特点是OTOA持续释放，释放的OTOA量超过PLA薄膜中初始含量的50%。OTOA释放曲线接近零级动力学，这有利于提供稳定的药物释放模式。所开发的PLA + 50% OTOA薄膜对具有多重耐药行为的 和 细菌菌株表现出强大而稳定的抗菌效果。所得的PLA + OTOA薄膜可用于多种生物医学和包装应用，包括伤口敷料和抗菌食品包装。
