Mruga Daryna, Berketa Kseniia, Sverstiuk Andrii, Martsenyuk Vasyl, Klos-Witkowska Aleksandra, Palianytsia Yurii, Dzyadevych Sergei, Soldatkin Oleksandr
Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 03680 Kyiv, Ukraine.
Institute of Biology and Medicine, Taras Shevchenko National University of Kyiv, 01003 Kyiv, Ukraine.
Sensors (Basel). 2024 Dec 10;24(24):7891. doi: 10.3390/s24247891.
This work presents the development of an amperometric biosensor for detecting aspartate aminotransferase (AST) activity in biological fluids using a platinum disk electrode as the working transducer. Optimal concentrations of substrates (aspartate, α-ketoglutarate) and the coenzyme (pyridoxal phosphate) were determined to ensure efficient biosensor operation. A semi-permeable poly-m-phenylenediamine membrane was applied to enhance selectivity against electroactive interferents. The biosensor demonstrated good stability (storage, continuous operation, and production reproducibility) and analytical performance (sensitivity 8.56 nA/min for 50 U/L AST, LOD 1 U/L, linear range 1-110 U/L). Testing with real samples showed a high correlation (R = 0.989) with spectrophotometric analysis, supporting its potential for further applications.
这项工作展示了一种用于检测生物体液中天冬氨酸转氨酶（AST）活性的安培型生物传感器的开发，该传感器使用铂盘电极作为工作换能器。确定了底物（天冬氨酸、α-酮戊二酸）和辅酶（磷酸吡哆醛）的最佳浓度，以确保生物传感器的高效运行。应用半透性聚间苯二胺膜来提高对电活性干扰物的选择性。该生物传感器表现出良好的稳定性（储存、连续运行和生产重现性）和分析性能（对50 U/L AST的灵敏度为8.56 nA/min，检测限为1 U/L，线性范围为1-110 U/L）。实际样品测试表明与分光光度分析具有高度相关性（R = 0.989），支持其进一步应用的潜力。
