Gu Jiayu, Zhang Xinqiao, Liu Tianlang, Guo Yunxue
Key Laboratory of Tropical Marine Bio-Resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510000, China.
University of Chinese Academy of Sciences, Beijing 100000, China.
Viruses. 2024 Nov 21;16(12):1816. doi: 10.3390/v16121816.
is a major global threat to human health, and phage therapy has emerged as a promising strategy for treating infections caused by multidrug-resistant pathogens. In this study, we isolated and characterized a lytic phage, PaTJ, from wastewater. PaTJ belongs to the phage family , and is featured by short latency (30 min) and large burst size (10 PFU per infected cell). Our investigation revealed that PaTJ utilizes the type IV Pili (T4P) as a receptor. Transcriptome analysis of PaTJ infected host at latent stage showed distinct expression patterns of PaTJ encoding genes involved in replication and structure assembly, without expression of the majority of toxic accessory genes responsible for phage release. In addition, host bacteria exhibited specific induction of host metabolism-related genes in response to the PaTJ's infection. Furthermore, our findings demonstrated the PaTJ's potential in degrading biofilms. This work sheds light on the multifaceted impact of this lytic phage PaTJ on , presenting potential applications in both gene expression modulation and biofilm management.
是对人类健康的重大全球威胁，噬菌体疗法已成为治疗由多重耐药病原体引起的感染的一种有前景的策略。在本研究中，我们从废水中分离并鉴定了一种裂解性噬菌体PaTJ。PaTJ属于噬菌体家族，其特点是潜伏期短（30分钟）和爆发量高（每个感染细胞产生10个噬菌斑形成单位）。我们的研究表明，PaTJ利用IV型菌毛（T4P）作为受体。对潜伏期受PaTJ感染的宿主进行转录组分析，结果显示参与复制和结构组装的PaTJ编码基因有明显的表达模式，而负责噬菌体释放的大多数有毒辅助基因未表达。此外，宿主细菌对PaTJ的感染表现出宿主代谢相关基因的特异性诱导。此外，我们的研究结果证明了PaTJ在降解生物膜方面的潜力。这项工作揭示了这种裂解性噬菌体PaTJ对……的多方面影响，在基因表达调控和生物膜管理方面都具有潜在应用。
