Rangel Caroline Lacorte, Fontes Silvia da Silva, Silva Marcus Vinicius de Mattos, Fernandes Jorlan, Mansur Janaina Figueira, Vilar Emmanuel Messias, da Costa-Neto Sócrates Fraga, Novaes Roberto Leonan Morim, Cordeiro-Estrela Pedro, Moratelli Ricardo, de Lemos Elba Regina Sampaio, Borges Ronaldo Mohana, Rodrigues-da-Silva Rodrigo Nunes, Oliveira Renata Carvalho de
Laboratório de Hantaviroses e Rickettsioses, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-900, Brazil.
Laboratório de Genômica Estrutural, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-971, Brazil.
Viruses. 2024 Nov 29;16(12):1857. doi: 10.3390/v16121857.
Hantaviruses are zoonotic pathogens associated with severe human diseases such as hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome. Despite the extensive study of rodent-borne hantaviruses, research on bat-associated hantaviruses remains limited. This study aimed to investigate the seroprevalence and cross-reactivity of neotropical bat samples with rodent- and bat-associated recombinant hantavirus nucleoproteins (rNPs) to improve hantavirus surveillance in the Brazilian Atlantic Forest. The studied bat population consisted of 336 blood samples collected over nearly a decade in five Brazilian states (Bahia, Rio de Janeiro, Santa Catarina, Paraná, and Minas Gerais). Antibodies were detected using IgG ELISA assays with rNPs from bat-borne (XSV) and (BRNV) and the rodent-borne hantaviruses (ANDV) and (SEOV). Results indicated a higher seroprevalence for the BRNV rNP (36.6%) compared to ANDV (7.4%), SEOV (5.7%), and XSV (0.6%). The high sensitivity of the BRNV rNP and the cross-reactivity observed with the ANDV rNP, the main protein used for serological tests in the Americas, indicates that BRNV rNP is a better antigen for the accurate detection of antibodies against hantaviruses in Brazilian bats. These findings underscore the presence of unknown hantaviruses antigenically similar to BRNV in Brazilian bat populations and highlight the urgent need for identifying better antigens for comprehensive hantavirus monitoring in bats.
汉坦病毒是与人的严重疾病相关的人畜共患病原体，如肾综合征出血热和汉坦病毒肺综合征。尽管对啮齿动物传播的汉坦病毒进行了广泛研究，但对蝙蝠相关汉坦病毒的研究仍然有限。本研究旨在调查新热带蝙蝠样本与啮齿动物和蝙蝠相关重组汉坦病毒核蛋白（rNP）的血清阳性率和交叉反应性，以改善巴西大西洋森林地区的汉坦病毒监测。所研究的蝙蝠种群包括在近十年间从巴西五个州（巴伊亚、里约热内卢、圣卡塔琳娜、巴拉那和米纳斯吉拉斯）采集的336份血液样本。使用来自蝙蝠传播的（XSV）和（BRNV）以及啮齿动物传播的汉坦病毒（ANDV）和（SEOV）的rNP通过IgG ELISA检测法检测抗体。结果表明，与ANDV（7.4%）、SEOV（5.7%）和XSV（0.6%）相比，BRNV rNP的血清阳性率更高（36.6%）。BRNV rNP的高敏感性以及与美洲血清学检测中使用的主要蛋白ANDV rNP观察到的交叉反应性表明，BRNV rNP是在巴西蝙蝠中准确检测抗汉坦病毒抗体的更好抗原。这些发现强调了巴西蝙蝠种群中存在与BRNV抗原相似的未知汉坦病毒，并突出了迫切需要鉴定更好的抗原以全面监测蝙蝠中的汉坦病毒。
