Saidu Muhammad Bello, Kúsz Norbert, Tsai Yu-Chi, Vágvölgyi Máté, Berkecz Róbert, Kókai Dávid, Burián Katalin, Hohmann Judit, Rédei Dóra
Department of Pharmacognosy, University of Szeged, Eötvös u. 6, 6720 Szeged, Hungary.
Institute of Pharmaceutical Analysis, University of Szeged, Somogyi u. 4, 6720 Szeged, Hungary.
Plants (Basel). 2022 Mar 13;11(6):764. doi: 10.3390/plants11060764.
Two undescribed compounds, 37-dihydroxy-24-methylenelanosta-8-ene-11-one () and neolignane deightonin () were isolated from the aerial parts of Croizat together with six known compounds, namely, kansenone (), euphorbol-7-one (), dehydrodiconiferyl diacetate (), marylaurencinol D (), scoparon (), and 3,4,3'-tri--methylellagic acid (). The structures of the isolated compounds were determined by HRESIMS, 1D (H, C JMOD) and 2D NMR (HSQC, HMBC, H-H COSY, NOESY) spectroscopic analysis, and by comparison of the assignments with literature data. The anti-herpes simplex virus type-2 activity of the isolated compounds were investigated by qRT-PCR assay on Vero cells after determining cytotoxic concentration 50% (CC). Compounds , , , and exhibited inhibitory effects with respective IC values of 7.05, 2.42, 11.73, and 0.032 µM. Scoparon () showed the strongest anti-HSV activity with a selectivity index of 10.93.
从克罗伊扎草的地上部分分离出两种未描述的化合物，3,7 - 二羟基 - 24 - 亚甲基羊毛甾 - 8 - 烯 - 11 - 酮（）和新木脂素代托宁（），以及六种已知化合物，即，蚕茧酮（）、大戟醇 - 7 - 酮（）、脱氢二松柏醇二乙酸酯（）、玛丽劳伦西诺醇D（）、滨蒿内酯（）和3,4,3'- 三 - O - 甲基鞣花酸（）。通过高分辨电喷雾电离质谱（HRESIMS）、一维（氢谱、碳谱、J 调制谱）和二维核磁共振（HSQC、HMBC、氢 - 氢相关谱、核Overhauser效应谱）光谱分析，并将化学位移数据与文献数据进行比较，确定了分离出的化合物的结构。在测定50%细胞毒性浓度（CC）后，通过对非洲绿猴肾细胞（Vero细胞）进行实时定量聚合酶链反应（qRT - PCR）测定，研究了分离出的化合物对2型单纯疱疹病毒的活性。化合物、、、表现出抑制作用，其各自的半数抑制浓度（IC）值分别为7.05、2.42、11.73和0.032 μM。滨蒿内酯（）显示出最强的抗单纯疱疹病毒活性，选择性指数为10.93。
