Shanmuga S S, Mariraj I, Rajeshkumar S, Dhanyaa M, Pradeep M
Nanobiomedicine lab, Centre for Global Health Research, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamilnadu, India.
Department of General Medicine, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamilnadu, India.
Med J Malaysia. 2025 Jan;80(Suppl 1):20-25.
The biomedical potential of silver nanoparticles (Ag NPs) synthesized with Zingiber officinale and Ocimum gratissimum herbal formulation was investigated in this study. The study aims to reveal their applications in various biomedical fields. The study evaluates the antioxidant, thrombolytic, and antimicrobial potential of Zingiber officinale and Ocimum gratissimum herbal formulation-mediated Ag NPs.
Biogenically synthesized silver nanoparticles (Ag NPs) from an herbal formulation containing Zingiber officinale and Ocimum gratissimum were tested at various concentrations using the DPPH (2,2- diphenyl-1-picrylhydrazyl) assay. The absorbance was measured at 517 nm to quantify DPPH free radicals. With Ag NP concentrations, the H2O2 test exhibited increased activity. This work evaluated the antibacterial activity of Ag NPs mediated by Zingiber officinale and Ocimum gratissimum against Staphylococcus aureus, Streptococcus mutans, Candida albicans, and Enterococcus faecalis.
The utilization of herbal formulations from Z. officinale and O. gratissimum to synthesize Ag NPs revealed considerable therapeutic effectiveness. At a concentration of 50 μl, the maximal inhibition was 76%, which is comparable in effectiveness to that of standard ascorbic acid. Significant blood clot dissolution was observed during thrombolytic testing at a concentration of 100μg/ml, indicating promising prospects for the treatment of thrombotic disorders. Nanoparticles dose-dependently inhibited E. faecalis, C. albicans, S. aureus, and S. mutans in antibacterial testing. These results show the potential of the nanoparticles as supplementary or alternative treatments to conventional antibiotics, particularly in light of the increasing prevalence of antibiotic resistance.
The further investigation of nanoparticles into their mechanisms and efficacy in therapeutic applications, positioning Zingiber officinale and Ocimum gratissimum formulation-mediated Ag NPs as viable candidates in developing antioxidant, thrombolytic, and antimicrobial treatments.
本研究调查了用生姜和罗勒草本配方合成的银纳米颗粒（Ag NPs）的生物医学潜力。该研究旨在揭示其在各种生物医学领域的应用。本研究评估了生姜和罗勒草本配方介导的Ag NPs的抗氧化、溶栓和抗菌潜力。
使用DPPH（2,2-二苯基-1-苦基肼）测定法，对含有生姜和罗勒的草本配方生物合成的银纳米颗粒（Ag NPs）在不同浓度下进行测试。在517nm处测量吸光度以量化DPPH自由基。随着Ag NP浓度的增加，H2O2测试显示活性增强。本研究评估了生姜和罗勒介导的Ag NPs对金黄色葡萄球菌、变形链球菌、白色念珠菌和粪肠球菌的抗菌活性。
利用生姜和罗勒的草本配方合成Ag NPs显示出相当大的治疗效果。在浓度为50μl时，最大抑制率为76%，其效果与标准抗坏血酸相当。在溶栓测试中，浓度为100μg/ml时观察到显著的血栓溶解，这表明在治疗血栓性疾病方面有广阔前景。在抗菌测试中，纳米颗粒对粪肠球菌、白色念珠菌、金黄色葡萄球菌和变形链球菌有剂量依赖性抑制作用。这些结果表明纳米颗粒作为传统抗生素补充或替代治疗的潜力，特别是鉴于抗生素耐药性的日益普遍。
进一步研究纳米颗粒在治疗应用中的作用机制和疗效，将生姜和罗勒配方介导的Ag NPs定位为开发抗氧化、溶栓和抗菌治疗的可行候选物。
