Guo Yonghui, Zhang Min, Luo Yingrui, Li Yingshi, Xu Yanxia, Wang Nisha
Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong Province, China.
Guangdong Provincial Clinical Research Center for Laboratory Medicine, Guangzhou, Guangdong Province, China.
Aging (Albany NY). 2024 Jul 8;17(1):203-216. doi: 10.18632/aging.205962.
Sarcoma (SARC) is a mesenchymal tumor which often responds poorly to systemic therapy. It is therefore important to look for possible biological markers that could tell the prognosis and the progression of SARC.
A combined evaluation of the Cancer Genome Atlas (TCGA) and genotypic tissue expression (GTEx) portal was used to analyzeLMNB2 expression level in different types of cancer. Kaplan-Meier survival analysis was performed to examine LMNB2 predictive value in over-all survival rate and disease-free survival rate. The association among LMNB2 expression level and immune cell infiltration, microsatellite instability (MSI) and tumor mutational burden (TMB) were analyzed. GO and KEGG enrichment analysis were performed to predicate LMNB2 biological functions. The biological function of LMNB2 was estimated by MTT and flow cytometry assay. Additionally, western blot assay was used to examine protein expression levels.
Increased LMNB2 expression was related with worsened cancer type-dependent survival. A relation between LMNB2 expression levels and immune cell infiltration was found. GO and KEGG enrichment analysis indicated that LMNB2 was involved in a series of pathways. Biology function assays revealed that down-regulation of LMNB2 impaired proliferation and cell cycle distribution. At the mechanical level, LMNB2 acts as a regulator of cyclinD1 and cyclinE1.
Altogether, these data suggest that LMNB2 may serve as a tumor promoter and could be a possible target for cancer therapy.
肉瘤（SARC）是一种间充质肿瘤，通常对全身治疗反应不佳。因此，寻找能够判断肉瘤预后和进展的潜在生物标志物很重要。
联合评估癌症基因组图谱（TCGA）和基因型组织表达（GTEx）数据库，分析不同类型癌症中LMNB2的表达水平。进行Kaplan-Meier生存分析，以检验LMNB2对总生存率和无病生存率的预测价值。分析LMNB2表达水平与免疫细胞浸润、微卫星不稳定性（MSI）和肿瘤突变负荷（TMB）之间的关联。进行GO和KEGG富集分析，以预测LMNB2的生物学功能。通过MTT和流式细胞术检测评估LMNB2的生物学功能。此外，使用蛋白质免疫印迹法检测蛋白质表达水平。
LMNB2表达增加与癌症类型依赖性生存恶化相关。发现LMNB2表达水平与免疫细胞浸润之间存在关联。GO和KEGG富集分析表明，LMNB2参与了一系列信号通路。生物学功能检测显示，LMNB2的下调会损害细胞增殖和细胞周期分布。在机制层面，LMNB2作为细胞周期蛋白D1和细胞周期蛋白E1的调节因子发挥作用。
总之，这些数据表明LMNB2可能作为肿瘤促进因子，并且可能成为癌症治疗的一个潜在靶点。
