LMNB2 是肝细胞癌的预后生物标志物，并与免疫浸润相关。

LMNB2 is a prognostic biomarker and correlated with immune infiltrates in hepatocellular carcinoma.

机构信息

Department of Emergency Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, China.

Department of Emergency Medicine, The First Affiliated Hospital of Anhui Medical University, Hefei, China.

出版信息

IUBMB Life. 2020 Dec;72(12):2672-2685. doi: 10.1002/iub.2408. Epub 2020 Nov 19.

DOI:10.1002/iub.2408

PMID:33211382

Abstract

BACKGROUND

Previous studies have suggested Lamin B2 (LMNB2) as an oncogene in lung cancer. However, the role of LMNB2 in hepatocellular carcinoma (HCC) remains unclear.

METHOD

The expression of LMNB2 was compared between HCC samples and non-tumor samples in multiple datasets. In addition, the prognostic value of LMNB2 in HCC was also investigated. Furthermore, the cBioPortal was utilized to analyze the genomic alternation of LMNB2 in HCC. Besides, co-expression genes and functional enrichment analysis were evaluated using LinkedOmics to determine the function of LMNB2. Finally, the correlation between LMNB2 and immune infiltration was assessed using Tumor Immune Estimation Resource (TIMER).

RESULTS

Elevated LMNB2 expression level was identified in HCC patients in multiple datasets. Moreover, increased levels of LMNB2 were associated with poor overall survival (OS) and disease-free survival (DFS). The functional enrichment analysis revealed that LMNB2 plays an essential role via the cell cycle pathway, spliceosome, hippo-signaling pathway, and metabolic pathways. Besides, copy number variation (CNV) and methylation were significantly associated with LMNB2 expression. Additionally, increased levels of LMNB2 were significantly associated with B cells, CD8+ T cells, CD4+ T cells, macrophages, neutrophils, and dendritic cells.

CONCLUSION

LMNB2 is a potential HCC prognostic and diagnostic biomarker.

摘要

背景

先前的研究表明 lamin B2（LMNB2）是肺癌的癌基因。然而，LMNB2 在肝细胞癌（HCC）中的作用尚不清楚。

方法

在多个数据集之间比较了 HCC 样本和非肿瘤样本中 LMNB2 的表达。此外，还研究了 LMNB2 在 HCC 中的预后价值。此外，利用 cBioPortal 分析了 HCC 中 LMNB2 的基因组改变。此外，使用 LinkedOmics 评估了共表达基因和功能富集分析，以确定 LMNB2 的功能。最后，使用 Tumor Immune Estimation Resource（TIMER）评估了 LMNB2 与免疫浸润的相关性。

结果

在多个数据集的 HCC 患者中，鉴定出 LMNB2 表达水平升高。此外，LMNB2 水平升高与总生存期（OS）和无病生存期（DFS）不良相关。功能富集分析表明，LMNB2 通过细胞周期途径、剪接体、 Hippo 信号通路和代谢途径发挥重要作用。此外，拷贝数变异（CNV）和甲基化与 LMNB2 表达显著相关。此外，LMNB2 水平升高与 B 细胞、CD8+T 细胞、CD4+T 细胞、巨噬细胞、中性粒细胞和树突状细胞显著相关。