Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
Department of Clinic and Surgery, School of Dentistry, Federal University of Alfenas, Alfenas, MG, Brazil.
Braz Dent J. 2021 Nov-Dec;32(6):107-114. doi: 10.1590/0103-6440202104103.
The purpose of the study was to investigate the association between single nucleotide polymorphisms (SNPs) in genes encoding estrogen receptors (ESR1 and ESR2, respectively) and delayed tooth emergence (DTE). This cross-sectional study was composed of biological unrelated children of both sexes, age ranging from 11 to 13 years old. DTE was defined when the successor primary tooth was still present in the oral cavity after its exfoliation time or the absence of the permanent tooth emergence into the oral cavity. Children were diagnosed with DTE when they had at least one delayed permanent tooth, according to age of exfoliation of each tooth proposed by The American Dental Association. Genomic DNA from saliva was used to evaluate the SNPs in ESR1 (rs9340799 and rs2234693) and ESR2 (rs1256049 and rs4986938) using Real-Time PCR. Chi-square or Fisher exact tests and Logistic Regression adjusted by age and gender were performed. SNP-SNP interaction was accessed by multifactor dimensionality reduction (MDR) analysis also adjusted by gender and age. The established alpha of this study was 5%. Among 537 included children, 296 (55%) were in the "DTE" group and the 241 (45%) were in the "Control" group. Age and gender were not statistically different among the groups (p>0.05). Genotype distribution of the SNPs rs9340799, rs2234693, rs1256049 and rs4986938 were not associated with DTE (p> 0.05). The models elected by MDR were not statistically significant either. Conclusions: The studied SNPs in ESR1 and ESR2 were not associated with permanent DTE.
本研究旨在探讨雌激素受体（ESR1 和 ESR2）基因编码单核苷酸多态性（SNP）与迟萌牙（DTE）之间的关联。这是一项横断面研究，包括生物上无亲缘关系的男女儿童，年龄在 11 至 13 岁之间。当继替恒牙在其脱落时间后仍存在于口腔中或恒牙未进入口腔时，定义为 DTE。根据美国牙科协会提出的每个牙齿脱落年龄，当儿童至少有一颗恒牙延迟萌出时，诊断为 DTE。使用 Real-Time PCR 从唾液中提取基因组 DNA，评估 ESR1（rs9340799 和 rs2234693）和 ESR2（rs1256049 和 rs4986938）中的 SNP。采用卡方检验或 Fisher 确切检验和 Logistic 回归分析，校正年龄和性别。采用多因素降维分析（MDR）评估 SNP-SNP 相互作用，同时校正性别和年龄。本研究设定的 alpha 值为 5%。在纳入的 537 名儿童中，296 名（55%）为“DTE”组，241 名（45%）为“对照组”。组间年龄和性别无统计学差异（p>0.05）。rs9340799、rs2234693、rs1256049 和 rs4986938 的 SNP 基因型分布与 DTE 无关（p>0.05）。MDR 选择的模型也没有统计学意义。结论：ESR1 和 ESR2 中的研究 SNP 与永久性 DTE 无关。
