Reis Caio Luiz Bitencourt, Guerra Kelem Cristina Cambraia Caproni, Barbosa Mariane Carolina Faria, Ferreira Fabricio Fernandes, de Lima Daniela Coelho, Segato Raquel Assed Bezerra, Almeida Ana Cláudia Pedreira de, Matsumoto Mirian Aiko Nakane, Baratto Filho Flares, Menezes Maria Angélica Hueb de, Küchler Erika Calvano, de Oliveira Daniela Silva Barroso
Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Brazil.
Department of Clinic and Surgery, School of Dentistry, Federal University of Alfenas, Brazil.
J Oral Biol Craniofac Res. 2024 Nov-Dec;14(6):700-705. doi: 10.1016/j.jobcr.2024.09.012. Epub 2024 Sep 27.
Genetic polymorphisms who disturb the mineral homeostasis during tooth development and eruption are candidate to clarify the molecular mechanisms involved in changes in the tooth eruption chronology. In this study, we evaluate whether the FokI (rs2228570) and BglI (rs739837) polymorphisms in the gene are associated with changes in the chronology of eruption of permanent teeth.
MATERIAL & METHOD: This cross-sectional study randomly included 353 biologically unrelated children, both sexes, without systemic impairment or syndromes and history of trauma during the primary dentition. One operator perform the oral clinical examination. The tooth was considered erupted if there was a visible minimum of any tooth surface emerging from the mucosa. Genomic DNA was extracted from buccal epithelial cells from saliva samples. Genotyping was performed by Real-Time Polymerase Chain Reactions using TaqMan® technology. The average of the total number of erupted permanent teeth between the genotypes was compared by the Mann-Whitney test and multivariate Generalized Linear Models (GLM) (α = 5 %). β values with Confidence Interval (CI) 95 % were calculated.
The heterozygous adenine-guanine genotype of the FokI significantly decreases the number of erupted permanent teeth (β = -1.15; CI 95 % = -2.22 to -0.07; p = 0.036). In the stratified analysis for maxillary and mandibular teeth, this genotype was associated with a decrease in the number of erupted maxillary permanent teeth (β = -0.65; CI 95 % = -1.22 to -0.09; p = 0.023). BglI was not associated with permanent teeth eruption.
The FokI, but not BglI, in the may delay the eruption of permanent teeth.
在牙齿发育和萌出过程中干扰矿物质稳态的基因多态性是阐明牙齿萌出时间变化所涉及分子机制的候选因素。在本研究中，我们评估该基因中的FokI（rs2228570）和BglI（rs739837）多态性是否与恒牙萌出时间的变化相关。
这项横断面研究随机纳入了353名无血缘关系的儿童，男女不限，无全身损害或综合征，且乳牙列期无外伤史。由一名操作人员进行口腔临床检查。如果有任何牙齿表面至少有可见部分从黏膜中萌出，则认为该牙齿已萌出。从唾液样本的颊上皮细胞中提取基因组DNA。使用TaqMan®技术通过实时聚合酶链反应进行基因分型。通过Mann-Whitney检验和多变量广义线性模型（GLM）（α = 5%）比较各基因型之间恒牙萌出总数的平均值。计算95%置信区间（CI）的β值。
FokI的杂合腺嘌呤 - 鸟嘌呤基因型显著减少了已萌出恒牙的数量（β = -1.15；95%CI = -2.22至 -0.07；p = 0.036）。在上颌牙和下颌牙的分层分析中，该基因型与上颌恒牙萌出数量的减少相关（β = -0.65；95%CI = -1.22至 -0.09；p = 0.023）。BglI与恒牙萌出无关。
该基因中的FokI而非BglI可能会延迟恒牙的萌出。
