Anaya-Ayala Javier E, Galicia-Vega Brenda J, Mejia-Cervantes Jacqueline, Ignacio-Alvarez Eleazar, Landero-Aguilar Ingrid A, Ferro-Flores Guillermina, Marquina-Castillo Brenda N, Gibbens-Bandala Brenda, Hinojosa Carlos A
Department of Surgery, Section of Vascular Surgery and Endovascular Therapy, Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran, Mexico City, Mexico.
Department of Nuclear Medicine, Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran, Mexico City, Mexico.
Pol J Radiol. 2024 Nov 20;89:e541-e548. doi: 10.5114/pjr/194210. eCollection 2024.
Monocyte chemoattractant protein-1 (MCP-1/CCL2) plays a key role for infiltration of monocytes/macrophages and studies have demonstrated that the MCP-1/C-C chemokine receptor 2 (CCR2) axis might be involved in the pathogenesis and progression of abdominal aortic aneurysms (AAA). Molecular imaging has shown potential for human clinical research studies. We evaluated the expression of CCR2 in patients with small AAA using single-photon emission computed tomography (SPECT) with the technetium-99m-6-hydrazinylnicotinoyl-C-C-chemokine receptor-2 ligand (Tc-HYNIC-CCR2-L).
A pilot study was performed to evaluate patients with small asymptomatic AAA. The equipment used was a Symbia T2 (Siemens, Germany), with radiolabeled Tc-HYNIC-CCR2-L. The SPECT uptake and activity were assessed and counted based on the region of interest (ROI), with nonparametric statistics being employed to compare the aneurysms site, left ventricle (Control 1) and regions with a nondiseased aorta (Control 2).
The three patients were male (100%) (mean age 81 years, and mean AAA maximum diameter of 40 mm, SD 3 mm). All patients tolerated the studies well. Images were obtained at one, two and four hours. The ROI mean value of the aneurysm site was 37,783 (SD 11,890), compared to the left ventricle (Control 1) 16,779 (SD 4397) (value = 0.0001); ROI for the nondiseased aortic region (Control 2) was significantly lower, 12,520 (SD 2141) (value = 0.0001).
Significant differences of CCR2 expression SPECT were found in the AAA site compared to the left ventricle and nondiseased aortic segments. The introduction of well-designed longitudinal studies with nuclear imaging modalities may assist in the molecular characterization of aneurysmal and rupture prediction.
单核细胞趋化蛋白-1（MCP-1/CCL2）在单核细胞/巨噬细胞浸润中起关键作用，研究表明MCP-1/C-C趋化因子受体2（CCR2）轴可能参与腹主动脉瘤（AAA）的发病机制和进展。分子成像已显示出在人体临床研究中的潜力。我们使用锝-99m-6-肼基烟酰基-C-C趋化因子受体-2配体（Tc-HYNIC-CCR2-L）单光子发射计算机断层扫描（SPECT）评估了小AAA患者中CCR2的表达。
进行了一项初步研究以评估无症状小AAA患者。使用的设备是Symbia T2（德国西门子公司），带有放射性标记的Tc-HYNIC-CCR2-L。基于感兴趣区域（ROI）评估并计算SPECT摄取和活性，采用非参数统计比较动脉瘤部位、左心室（对照1）和无病变主动脉区域（对照2）。
三名患者均为男性（100%）（平均年龄81岁，AAA最大平均直径40 mm，标准差3 mm）。所有患者对研究耐受性良好。在1小时、2小时和4小时获取图像。动脉瘤部位的ROI平均值为37,783（标准差11,890），相比之下左心室（对照1）为16,779（标准差4397）（值 = 0.0001）；无病变主动脉区域（对照2）的ROI显著更低，为12,520（标准差2141）（值 = 0.0001）。
与左心室和无病变主动脉段相比，在AAA部位发现CCR2表达SPECT存在显著差异。引入设计良好的核成像方式纵向研究可能有助于动脉瘤的分子特征分析和破裂预测。
