Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
Poult Sci. 2020 Oct;99(10):4947-4957. doi: 10.1016/j.psj.2020.06.033. Epub 2020 Jul 4.
The objective of this experiment was to determine total amino acid (TAA) content, standardized ileal digestibility (SID) of crude protein, and standardized ileal amino acid digestibility in 9 sources of soybean meal (SBM) of different origin and to subsequently establish equations for predicting the TAA content and concentration of standardized ileal digestible amino acids (SIDAA) based on their protein content and other proximate components. Concentration of SIDAA of the samples was also predicted using TAA values. A total of 160 1-day-old male broiler chicks were randomly assigned to 10 dietary treatments consisted of 9 semipurified diets containing one SBM (200 g of crude protein/kg) as the only source of dietary amino acid (AA) and one N-free diet to determine endogenous ileal AA flow. The birds were fed with a standard diet from 0 to 18 D of age, and experimental diets were fed from 19 to 24 D of age. The fitness of the models of the study was tested using the adjusted coefficient of determination (R) value, P-value regression and coefficients, and standard error of prediction (SEP). The coefficient of SID for Lys and Cys among SBM varied from 86.7 to 96.3 and 74.1 to 89.3, respectively, with significant difference (P < 0.05). In equations based on protein content, the adjusted R value ranged from 40.7 (Ile) to 99.6 (Met) and 37.2 (Met + Cys) to 99.6 (Met) for TAA content and concentration of SIDAA, respectively. Inclusion of other proximate components of test samples (e.g., crude fiber, neutral detergent fiber, acid detergent fiber, ash, gross energy, and so on) into the regression equation increased the adjusted R value and decreased the SEP. The results of linear regression revealed that it is possible to satisfactorily estimate the TAA content and concentration of SIDAA of SBM through its protein content and other proximate components, but the prediction equations based on other proximate components were more accurate in terms of reflecting the measured results; however, additional time and costs were associated with this approach. It is also possible to estimate the concentration of SIDAA through TAA values with reasonable accuracy and lower SEP.
本实验旨在测定 9 种不同来源的大豆粕(SBM)的总氨基酸(TAA)含量、标准回肠可消化粗蛋白(SID)和标准回肠可消化氨基酸(SIDAA)浓度,并随后建立基于其蛋白含量和其他近似成分预测 TAA 含量和 SIDAA 浓度的方程。还使用 TAA 值预测了样品的 SIDAA 浓度。总共 160 只 1 日龄雄性肉鸡随机分配到 10 种饲粮处理中,其中 9 种半纯化饲粮含有一种 SBM(200g 粗蛋白/kg)作为唯一的饲粮氨基酸(AA)来源,一种无氮饲粮用于测定回肠内源 AA 流量。0-18 日龄时,雏鸡饲喂标准饲粮,19-24 日龄时,饲喂试验饲粮。使用调整后的决定系数(R)值、P 值回归和系数以及预测标准误差(SEP)来检验研究模型的拟合度。SBM 中 Lys 和 Cys 的 SID 系数分别在 86.7-96.3 和 74.1-89.3 之间变化,差异显著(P<0.05)。基于蛋白含量的方程中,TAA 含量和 SIDAA 浓度的调整 R 值分别在 40.7(Ile)到 99.6(Met)和 37.2(Met+Cys)到 99.6(Met)之间。将测试样品的其他近似成分(如粗纤维、中性洗涤纤维、酸性洗涤纤维、灰分、总能等)纳入回归方程可提高调整后的 R 值并降低 SEP。线性回归的结果表明,通过 SBM 的蛋白含量和其他近似成分可以很好地预测 TAA 含量和 SIDAA 浓度,但基于其他近似成分的预测方程在反映实测结果方面更为准确;然而,这一方法需要更多的时间和成本。也可以通过 TAA 值以合理的精度和较低的 SEP 来估计 SIDAA 浓度。