Diakaki Makrina, Andreo Jimenez Beatriz, de Lange Ezra, Butterbach Patrick, van der Heijden Liesbeth, Köhl Jürgen, de Boer Wietse, Postma Joeke
Wageningen Plant Research, Wageningen University and Research, 6708 PB Wageningen, The Netherlands.
Soil Biology Group, Wageningen University and Research, 6708 PB Wageningen, The Netherlands.
FEMS Microbiol Ecol. 2025 Jan 28;101(2). doi: 10.1093/femsec/fiaf004.
Recently we demonstrated that the seed microbiome of certain spinach (Spinacia oleracea) seed lots can confer disease suppression against Globisporangium ultimum damping-off (previously known as Pythium ultimum). We hypothesized that differences in the microbial community composition of spinach seed lots correlate with the levels of damping-off suppressiveness of each seed lot. Here, we show that a large proportion of variance in seed-associated bacterial (16S) and fungal (Internal Transcribed Spacer 1) amplicon sequences was explained by seed lot identity, while 9.8% of bacterial and 7.1% of fungal community variance correlated with disease suppression. More specifically, a higher relative abundance of basidiomycetous dimorphic yeasts such as Vishniacozyma, Filobasidium, and Papiliotrema and of the bacterial genus Massilia was a key feature of suppressive seed microbiomes. We suggest that the abundance of these genera is indicative of seed lot suppressive potential. Seed processing and treatment can become more targeted with indicator taxa being used to evaluate the presence of beneficial seed-associated microbial functions. This process, in turn, could contribute to the sustainable management of seedling diseases. Finally, this study highlights the ubiquity of yeasts in spinach seed microbiota and their potential beneficial roles for seed health.
最近我们证明，某些菠菜（Spinacia oleracea）种子批次的种子微生物群能够抑制终极球囊霉（以前称为终极腐霉）引起的猝倒病。我们假设菠菜种子批次的微生物群落组成差异与每个种子批次的猝倒病抑制水平相关。在此，我们表明，种子相关细菌（16S）和真菌（内转录间隔区1）扩增子序列的很大一部分变异可由种子批次身份解释，而9.8%的细菌群落变异和7.1%的真菌群落变异与病害抑制相关。更具体地说，担子菌双态酵母（如威尼克酵母、丝孢酵母和蝶形酵母）以及马赛菌属细菌的相对丰度较高是抑制性种子微生物群的一个关键特征。我们认为这些属的丰度表明了种子批次的抑制潜力。种子加工和处理可以更有针对性，利用指示分类群来评估有益的种子相关微生物功能的存在。反过来，这一过程有助于幼苗病害的可持续管理。最后，本研究强调了酵母在菠菜种子微生物群中的普遍性及其对种子健康的潜在有益作用。
