Uusitalo R J, Stjernschantz J, Mahlberg K A, Gregerson D S, Uusitalo H, Tallberg T, Palkama A
Br J Ophthalmol. 1985 Mar;69(3):212-6. doi: 10.1136/bjo.69.3.212.
Bovine S-antigen was purified by gel filtration and ion exchange chromatography according to previously described techniques. An enzyme linked immunosorbent assay (ELISA) using antiserum to bovine S-antigen raised in guinea-pigs was employed to detect S-antigen in the chromatographic fractions. The purity of the S-antigen was determined by SDS-PAGE electrophoresis, where a single band was found. The purified S-antigen in microgram quantities together with Freund's complete adjuvant induced uveitis in rats two weeks after injection into the foot pad. Serum samples from children suffering from chronic uveitis and healthy children were tested for antibodies to S-antigen by the ELISA. A statistically significant difference in the level of specific antibodies between patients and controls was found. There was no clear-cut correlation between the severity of uveitis and antibody titre, but cases with retinal involvement and aggressive uveitis all showed definite elevations of antibodies to S-antigen.
按照先前描述的技术，通过凝胶过滤和离子交换色谱法纯化牛S抗原。使用豚鼠体内产生的抗牛S抗原抗血清的酶联免疫吸附测定（ELISA）来检测色谱级分中的S抗原。通过SDS-PAGE电泳测定S抗原的纯度，结果发现只有一条带。将微克量的纯化S抗原与弗氏完全佐剂一起注射到大鼠脚垫中，两周后诱发大鼠葡萄膜炎。通过ELISA检测患有慢性葡萄膜炎的儿童和健康儿童的血清样本中抗S抗原的抗体。发现患者和对照组之间特异性抗体水平存在统计学上的显著差异。葡萄膜炎的严重程度与抗体滴度之间没有明确的相关性，但视网膜受累和侵袭性葡萄膜炎的病例均显示抗S抗原抗体明显升高。
