Van Tuyen V, Faure J P, Thillaye B, de Kozak Y, Fortier B
Curr Eye Res. 1982;2(1):7-12. doi: 10.3109/02713688208998375.
Enzyme-linked immunosorbent assay was applied to the determination of the serum IgG antibody contents in rats immunized with the organ-specific autoantigen (S antigen) of the retina. Optimal conditions (i.e. S antigen concentration, serum and conjugate dilutions, enzymatic reaction time) were determined. The assay required only a single serum dilution and was well reproducible. It was very sensitive, allowing the detection of low antibody contents in animals immunized with 1 microgram of S antigen. The time course of the antibody response and its variations according to the dose and the species of the immunizing S antigen were considered.
采用酶联免疫吸附测定法测定用视网膜器官特异性自身抗原（S抗原）免疫的大鼠血清IgG抗体含量。确定了最佳条件（即S抗原浓度、血清和结合物稀释度、酶促反应时间）。该测定仅需对血清进行一次稀释，且重复性良好。它非常灵敏，能够检测出用1微克S抗原免疫的动物体内的低抗体含量。研究了抗体反应的时间进程及其根据免疫S抗原的剂量和种类的变化情况。
