Savitri Irma Josefina, Suprarahardiani Safira Alif, Ulinnuha Laila Kamilatu, Prahasanti Chiquita, Wijaksana I Komang Evan, Ridwan Rini Devijanti, E Diah Savitri, Aljunaid Mohammed Ahmed, Syed Sadatullah, Abullais Shahabe Saquib
Department of Periodontology, Faculty of Dental Medicine, Universitas Airlangga Surabaya, Indonesia.
Research Center for Biomaterial and Tissue Engineering, Universitas Airlangga Surabaya, Indonesia.
Int J Med Sci. 2025 Jan 1;22(2):318-327. doi: 10.7150/ijms.99156. eCollection 2025.
: Live microorganisms, named probiotics, can improve overall physical well-being, particularly the oral cavity's health. , a popular probiotic, can influence the immune response by increasing the number of macrophages and plasma cells that play a role in traumatic ulcer healing. : To determine the expression of tumor necrosis factor-alpha (TNF-α) and the varied number of plasma cells and macrophages on a traumatic ulcer animal model treated with topical or systemic administration of a probiotic : Thirty-six healthy, 2-3-month-old male weighing 175-250 gram, were designed into control and topical and systemic administration probiotic groups. The control group was treated with Aquadest 20 uL/20 gr, while topical probiotic and systemic administration probiotic groups were treated with 10,9x10 cells/kg, respectively. A heated round burnisher tip was used to traumatize the inferior incisive fornix labial area. After 3 or 7 days, the animal models were terminated. Immunohistochemical examination, Hematoxylin eosin staining, and statistical analysis were performed to analyze the expression of TNF-α and the number of plasma cells and macrophages. : The Mann-Whitney and Tukey HSD tests indicated significant differences ( < 0.05) in the results for the three groups. It was observed that topical administration provides more remarkable results than systemic administration for the expression of TNF-α, the number of plasma cells, and the number of macrophages. : Topical administration of demonstrates better results than systemic administration for healing traumatic ulcers.
被称为益生菌的活微生物可以改善整体身体健康，尤其是口腔健康。一种常见的益生菌可以通过增加在创伤性溃疡愈合中起作用的巨噬细胞和浆细胞数量来影响免疫反应。目的：确定在局部或全身给予益生菌治疗的创伤性溃疡动物模型中肿瘤坏死因子-α（TNF-α）的表达以及浆细胞和巨噬细胞数量的变化。方法：将36只健康的、2 - 3个月大、体重175 - 250克的雄性动物分为对照组、局部给予益生菌组和全身给予益生菌组。对照组用20微升/20克蒸馏水治疗，而局部给予益生菌组和全身给予益生菌组分别用10,9×10个细胞/千克治疗。使用加热的圆形抛光器尖端损伤下颌切牙唇侧穹窿区。3天或7天后，处死动物模型。进行免疫组织化学检查、苏木精-伊红染色和统计分析，以分析TNF-α的表达以及浆细胞和巨噬细胞的数量。结果：曼-惠特尼检验和图基HSD检验表明三组结果存在显著差异（P < 0.05）。观察到在TNF-α的表达、浆细胞数量和巨噬细胞数量方面，局部给药比全身给药效果更显著。结论：局部给予益生菌治疗创伤性溃疡比全身给药效果更好。
