Kusumaningsih Tuti, Irmawati Anis, Ernawati Diah Savitri, Prahasanti Chiquita, Aljunaid Mohammed, Amelia Sarah
Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
Vet World. 2021 May;14(5):1279-1283. doi: 10.14202/vetworld.2021.1279-1283. Epub 2021 May 23.
The use of drugs as a therapy for traumatic ulcers may lead to drug resistance and other side effects. Shirota can affect the number of fibroblasts and blood vessels in wound healing. The aim of this study was to investigate the difference in the number of fibroblast cells and blood vessels after the topical and systemic administration of Shirota probiotics in Wistar rats with traumatic ulcer.
Overall, 36 healthy male Wistar rats aged 2-3 months old and weighing 175-250 g in body weight were used as a sample. Traumatic ulcer was made on the labial fornix incisive inferior. The subject rats were divided into groups: (1) A control group over 3 days, (2) a group that used distilled water over 7 days, (3) a group that underwent topical treatment over 3 days, (4) a group that used probiotics administered topically over 7 days, (5) a group that underwent systemic treatment over 3 days, and (6) a group that took oral probiotics for the traumatic ulcers over 7 days. The number of fibroblasts and blood vessels was observed through a hematoxylin-eosin examination.
Based on the results of the study, a significant difference was observed in the number of fibroblasts (p=0.00) and blood vessels (p=0.018) in the 3-day topical group that underwent a 3-day systemic administration of probiotics compared with the number of fibroblast cells in the 7-day topical group and 7-day systemic group (p=0.00).
Overall, significant differences were observed in the number of fibroblasts and blood vessels in Wistar rats with traumatic ulcer after undergoing the topical and systemic administration of Shirota probiotics.
使用药物治疗创伤性溃疡可能会导致耐药性及其他副作用。施罗塔（Shirota）益生菌可影响伤口愈合过程中纤维母细胞和血管的数量。本研究旨在探讨对患有创伤性溃疡的Wistar大鼠局部和全身施用施罗塔益生菌后，其纤维母细胞和血管数量的差异。
总共选用36只2至3月龄、体重175至250克的健康雄性Wistar大鼠作为样本。在切牙下唇穹窿处制造创伤性溃疡。将受试大鼠分为以下几组：（1）3天的对照组；（2）7天使用蒸馏水的组；（3）3天进行局部治疗的组；（4）7天局部施用益生菌的组；（5）3天进行全身治疗的组；（6）7天口服益生菌治疗创伤性溃疡的组。通过苏木精 - 伊红染色检查观察纤维母细胞和血管的数量。
基于研究结果，与7天局部施用组和7天全身施用组的纤维母细胞数量相比，3天全身施用益生菌的3天局部施用组在纤维母细胞数量（p = 0.00）和血管数量（p = 0.018）上存在显著差异（p = 0.00）。
总体而言，对患有创伤性溃疡的Wistar大鼠局部和全身施用施罗塔益生菌后，其纤维母细胞和血管数量存在显著差异。
