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气-水界面处的磷脂表面双层膜。II. 二肉豆蔻酰磷脂酰胆碱表面双层膜的水渗透性。

Phospholipid surface bilayers at the air-water interface. II. Water permeability of dimyristoylphosphatidylcholine surface bilayers.

作者信息

Ginsberg L, Gershfeld N L

出版信息

Biophys J. 1985 Feb;47(2 Pt 1):211-5. doi: 10.1016/s0006-3495(85)83893-5.

Abstract

Dispersions of dimyristoylphosphatidylcholine (DMPC) in water have been reported to form a structure at 29 degrees C at the equilibrium air/water surface with a molecular density equal to that of a typical bilayer. In this study, the water permeability of this structure has been evaluated by measuring the rate of water evaporation from DMPC dispersions in water in the temperature range where the surface film density exceeds that of a monolayer. Evaporation rates for the lipid dispersions did not deviate from those for lipid-free systems throughout the entire temperature range examined (20-35 degrees C) except at 29 degrees C, where a barrier to evaporation was detected. This strengthens the view that the structure that forms at this temperature has the properties of a typical bilayer.

摘要

据报道,二肉豆蔻酰磷脂酰胆碱(DMPC)在水中的分散体在29摄氏度时于平衡空气/水表面形成一种结构,其分子密度与典型双层膜的分子密度相等。在本研究中,通过测量在表面膜密度超过单层膜密度的温度范围内,DMPC在水中的分散体的水分蒸发速率,对该结构的水渗透性进行了评估。除了在29摄氏度检测到蒸发屏障外,在所研究的整个温度范围(20 - 35摄氏度)内,脂质分散体的蒸发速率与无脂质体系的蒸发速率没有偏差。这强化了这样一种观点,即在该温度下形成的结构具有典型双层膜的性质。

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