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气-水界面处的磷脂表面双层膜。III. 表面双层膜形成与细胞膜中脂质双层组装之间的关系。

Phospholipid surface bilayers at the air-water interface. III. Relation between surface bilayer formation and lipid bilayer assembly in cell membranes.

作者信息

Gershfeld N L

出版信息

Biophys J. 1986 Sep;50(3):457-61. doi: 10.1016/S0006-3495(86)83482-8.

Abstract

Lipid bilayer assembly in cell membranes has been simulated with total lipid extracts from human red blood cells and from mesophilic and thermophilic bacteria grown at several temperatures. Aqueous dispersions of these natural lipid mixtures form surface bilayers, a single bimolecular lipid state, but only at the growth temperature of the source organism. Thus, a single isolated bilayer state forms spontaneously in vitro from lipids that are available in vivo at the growth temperature of the cell. Surface bilayers form at a specific temperature that is a function of hydrocarbon chain length and degree of fatty acid unsaturation of the phospholipids; this property is proposed as an essential element in the control of membrane lipid composition.

摘要

细胞膜中的脂质双层组装已通过从人类红细胞以及在几种温度下生长的嗜温菌和嗜热菌中提取的总脂质进行了模拟。这些天然脂质混合物的水分散体形成表面双层,即单一的双分子脂质状态,但仅在源生物体的生长温度下形成。因此,在体外,单一分离的双层状态可由细胞生长温度下体内可用的脂质自发形成。表面双层在特定温度下形成,该温度是磷脂烃链长度和脂肪酸不饱和度的函数;这一特性被认为是控制膜脂质组成的一个基本要素。

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