干燥取向样品中细菌视紫红质光循环的阿累尼乌斯参数。
Arrhenius parameters of the bacteriorhodopsin photocycle in dried oriented samples.
作者信息
Váró G, Keszthelyi L
出版信息
Biophys J. 1985 Feb;47(2 Pt 1):243-6. doi: 10.1016/s0006-3495(85)83897-2.
Abstract
In dried oriented samples of purple membranes isolated from Halobacterium halobium the Arrhenius parameters of the photocycle showed an abrupt change at a water content of approximately 80 H2O molecules per bacteriorhodopsin molecule. This makes probable the existence of a water-dependent conformational change of the protein. This result underlines the importance of water in the proton-conduction mechanism inside the protein. The effect of the external electric potential on the rate constants of the photoelectric signals was also measured. The data demonstrate that the membrane potential affects the steps of the proton transport during the photocycle.
摘要
在从嗜盐菌中分离出的紫色膜的干燥定向样品中，光循环的阿累尼乌斯参数在每个细菌视紫红质分子约含80个水分子的含水量处出现了突然变化。这使得蛋白质存在依赖于水的构象变化成为可能。该结果强调了水在蛋白质内部质子传导机制中的重要性。还测量了外部电势对光电信号速率常数的影响。数据表明，膜电位影响光循环期间质子运输的步骤。
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