Pu Xujun, Niu Meirong, Fan Xuejing, Sun Liping, Gu Ying, Wang Shuo
Faculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China.
Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin 300071, China.
J Agric Food Chem. 2025 Jan 22;73(3):2186-2196. doi: 10.1021/acs.jafc.4c10516. Epub 2025 Jan 9.
Conquering surface fouling of sensors caused by nonspecific adsorption and accumulation of foulants in a food matrix is of significance in accurate food safety analysis. Herein, an antifouling electrochemical aptasensor based on a Y-shaped peptide and nanoporous gold (NPG) for aflatoxin B1 detection in milk, tofu, and rice flour was proposed. The self-designed Y-shaped peptide involves an anchoring segment (-C), a support structure (-PPPP-), and an antifouling domain with two branches (-EK(KSRE)DER-) inspired by two bioactive peptides. NPG offers not only good electroconductibility but also anchor sites for peptides and aptamers. The antifouling surface can effectively resist fouling from single protein and carbohydrate solutions and even real food. The proposed aptasensor achieves sensitive detection with a low limit of detection of 0.26 pg mL (linear range: 0.001-10 ng mL) and accurate analysis with minimal sample pretreatment (recoveries: 91.0-110.8%), offering a potential sensing platform to lower matrix interference.
克服由食品基质中污垢的非特异性吸附和积累引起的传感器表面污染对于准确的食品安全分析具有重要意义。在此，提出了一种基于Y形肽和纳米多孔金（NPG）的抗污染电化学适体传感器，用于检测牛奶、豆腐和米粉中的黄曲霉毒素B1。自行设计的Y形肽包括一个锚定片段（-C）、一个支撑结构（-PPPP-）和一个受两种生物活性肽启发的具有两个分支的抗污染结构域（-EK(KSRE)DER-）。NPG不仅具有良好的导电性，还为肽和适体提供锚定位点。该抗污染表面可以有效抵抗来自单一蛋白质和碳水化合物溶液甚至实际食品的污染。所提出的适体传感器实现了灵敏检测，检测限低至0.26 pg mL（线性范围：0.001 - 10 ng mL），并且在最小化样品预处理的情况下进行准确分析（回收率：91.0 - 110.8%），为降低基质干扰提供了一个潜在的传感平台。
