Cary P L, Johnson C A, Whitter P D, Parker J W
Clin Chem. 1985 Apr;31(4):605-8.
Determination of cholesterol by a radiative energy attenuation (REA) technique was evaluated and compared with results obtained by the Boehringer Mannheim High Performance Cholesterol Assay and the Du Pont aca. Within-assay and between-assay CVs for the REA method, for two sets of controls, were both less than 5%. We observed no interference with lipemic samples. Analytical recovery averaged 102.8%. We used all three methods for parallel determinations of 217 patients' samples containing a wide range of cholesterol concentrations. Linear regression analysis of the REA results vs those of the comparison methods were as follows: REA = 1.03 Boehringer - 0.072 (r = 0.993) and REA = 1.02 aca - 0.048 (r = 0.995). We also discuss bilirubin interference with the REA method for cholesterol.
对采用辐射能量衰减（REA）技术测定胆固醇的方法进行了评估，并与用勃林格殷格翰高效胆固醇测定法和杜邦aca法所得结果进行了比较。对于两组对照品，REA法的批内和批间变异系数均小于5%。我们观察到该方法对脂血样本无干扰。分析回收率平均为102.8%。我们用这三种方法对217例胆固醇浓度范围广泛的患者样本进行了平行测定。REA结果与比较方法结果的线性回归分析如下：REA = 1.03×勃林格殷格翰法结果 - 0.072（r = 0.993），REA = 1.02×aca法结果 - 0.048（r = 0.995）。我们还讨论了胆红素对REA法测定胆固醇的干扰。
